Nguyen T D, Bottreau E, Bernard S, Lantier I, Aynaud J M
J Gen Virol. 1986 May;67 ( Pt 5):939-43. doi: 10.1099/0022-1317-67-5-939.
Secretory IgA (sIgA) and IgG from porcine milk and serum, respectively, [3H]uridine-labelled virus, swine testis and pig kidney cell lines were used to examine the neutralized virus-cell interaction. Transmissible gastroenteritis virus (TGEV), 99.99% neutralized by immunoglobulin, was able to attach to the cells. Moreover, sIgA enhanced virus attachment. However, the neutralized virus was unable to enter cells, as demonstrated by the action of proteinase K which removed it from the cell surface. It was also found that pre-attached virus was still neutralizable and that IgG and sIgA had similar TGEV-neutralizing capacities.
分别来自猪乳和血清的分泌型IgA（sIgA）和IgG、[3H]尿苷标记的病毒、猪睾丸和猪肾细胞系被用于检测中和病毒与细胞的相互作用。传染性胃肠炎病毒（TGEV），99.99%被免疫球蛋白中和，能够附着于细胞。此外，sIgA增强了病毒的附着。然而，蛋白酶K的作用表明被中和的病毒无法进入细胞，蛋白酶K将其从细胞表面去除。还发现预先附着的病毒仍可被中和，并且IgG和sIgA具有相似的TGEV中和能力。
