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1
Serum albumin treatment to eliminate false-positive results with laboratory rodent specimens tested by rotazyme enzyme-linked immunosorbent assay.用血清白蛋白处理以消除通过Rotazyme酶联免疫吸附测定法检测的实验啮齿动物标本的假阳性结果。
J Clin Microbiol. 1987 Nov;25(11):2239-40. doi: 10.1128/jcm.25.11.2239-2240.1987.
2
Identification of nonspecific reactions in laboratory rodent specimens tested by Rotazyme rotavirus ELISA.通过Rotazyme轮状病毒酶联免疫吸附测定法检测实验室啮齿动物标本中的非特异性反应的鉴定。
Lab Anim Sci. 1988 Jun;38(3):273-8.
3
Rotazyme assay in neonates without diarrhea. Results of screening survey and preliminary analysis of false positive specimens.新生儿无腹泻时的Rotazyme检测。筛查调查结果及假阳性标本的初步分析。
Am J Clin Pathol. 1985 Dec;84(6):748-51. doi: 10.1093/ajcp/84.6.748.
4
Evaluation of assay systems for the detection of rotavirus in stool specimens.
Diagn Microbiol Infect Dis. 1989 Mar-Apr;12(2):139-41. doi: 10.1016/0732-8893(89)90004-7.
5
Enzyme-linked immunosorbent assay for the detection of human rotavirus in stools.用于检测粪便中人类轮状病毒的酶联免疫吸附测定法。
J Virol Methods. 1983 Aug;7(2):65-72. doi: 10.1016/0166-0934(83)90093-9.
6
Comparison of nine commercial immunoassays for the detection of rotavirus in fecal specimens.九种用于检测粪便标本中轮状病毒的商用免疫测定法的比较。
J Clin Microbiol. 1988 Sep;26(9):1630-4. doi: 10.1128/jcm.26.9.1630-1634.1988.
7
Detection of rotavirus with a new polyclonal antibody enzyme immunoassay (Rotazyme II) and a commercial latex agglutination test (Rotalex): comparison with a monoclonal antibody enzyme immunoassay.采用新型多克隆抗体酶免疫测定法（Rotazyme II）和市售乳胶凝集试验（Rotalex）检测轮状病毒：与单克隆抗体酶免疫测定法的比较
J Clin Microbiol. 1986 Feb;23(2):226-9. doi: 10.1128/jcm.23.2.226-229.1986.
8
Evaluation of two commercial kits for rapid detection of human rotavirus in feces: Rotalex, a latex agglutination test and Rotavirus ELISA Kit.两种用于粪便中人类轮状病毒快速检测的商业试剂盒评估：乳胶凝集试验Rotalex和轮状病毒酶联免疫吸附测定试剂盒。
Acta Pathol Microbiol Immunol Scand B. 1985 Apr;93(2):159-60. doi: 10.1111/j.1699-0463.1985.tb02869.x.
9
Comparison of four latex agglutination (LA) and three enzyme-linked immunosorbent assays (ELISA) for the detection of rotavirus in fecal specimens.
Am J Clin Pathol. 1989 Nov;92(5):637-43. doi: 10.1093/ajcp/92.5.637.
10
Detection of rotavirus in faecal specimens with a monoclonal antibody enzyme-linked immunosorbent assay: comparison with polyclonal antibody enzyme immuno-assays and a latex agglutination test.用单克隆抗体酶联免疫吸附测定法检测粪便标本中的轮状病毒：与多克隆抗体酶免疫测定法和乳胶凝集试验的比较
Comp Immunol Microbiol Infect Dis. 1989;12(4):95-104. doi: 10.1016/0147-9571(89)90058-1.

本文引用的文献

1
Enzyme immunoassays for the detection of infectious antigens in body fluids: current limitations and future prospects.用于检测体液中感染性抗原的酶免疫测定法：当前局限性与未来前景
Rev Infect Dis. 1982 Jan-Feb;4(1):35-68. doi: 10.1093/clinids/4.1.35.
2
Enzyme immunoassays for the diagnosis of viral infections.用于诊断病毒感染的酶免疫测定法。
Ann N Y Acad Sci. 1983;420:381-90. doi: 10.1111/j.1749-6632.1983.tb22227.x.
3
Unreliability of Rotazyme ELISA test in neonates.新生儿Rotazyme酶联免疫吸附测定试验的不可靠性。
J Pediatr. 1983 Aug;103(2):259-62. doi: 10.1016/s0022-3476(83)80361-8.
4
Comparison of an enzyme immunoassay with electron microscopic procedures for detecting rotavirus.酶免疫测定法与电子显微镜检测程序在轮状病毒检测中的比较。
J Clin Microbiol. 1982 May;15(5):938-44. doi: 10.1128/jcm.15.5.938-944.1982.
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Synergistic rotavirus and Escherichia coli diarrheal infection of mice.小鼠轮状病毒与大肠杆菌协同性腹泻感染
Infect Immun. 1985 Feb;47(2):573-4. doi: 10.1128/iai.47.2.573-574.1985.
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Cultivation and characterization of three strains of murine rotavirus.三株鼠轮状病毒的培养与特性分析
J Virol. 1986 Feb;57(2):585-90. doi: 10.1128/JVI.57.2.585-590.1986.
7
Examination of the Rotazyme II enzyme immunoassay for the diagnosis of rotavirus gastroenteritis.用于诊断轮状病毒肠胃炎的Rotazyme II酶免疫测定法的检测
J Clin Microbiol. 1985 Sep;22(3):462-4. doi: 10.1128/jcm.22.3.462-464.1985.
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Rotavirus-associated diarrhea in a commercial rabbitry.
Lab Anim Sci. 1986 Apr;36(2):149-52.
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Analysis of nonspecific reactions in enzyme-linked immunosorbent assay testing for human rotavirus.人轮状病毒酶联免疫吸附测定中非特异性反应的分析
J Clin Microbiol. 1979 Nov;10(5):703-7. doi: 10.1128/jcm.10.5.703-707.1979.
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Rotaviruses.轮状病毒
J Gen Virol. 1978 Jul;40(1):1-18. doi: 10.1099/0022-1317-40-1-1.

用血清白蛋白处理以消除通过Rotazyme酶联免疫吸附测定法检测的实验啮齿动物标本的假阳性结果。

Serum albumin treatment to eliminate false-positive results with laboratory rodent specimens tested by rotazyme enzyme-linked immunosorbent assay.

作者信息

Jure M N, Morse S S, Stark D M

机构信息

Laboratory Animal Research Center, Rockefeller University, New York, New York 10021-6399.

出版信息

J Clin Microbiol. 1987 Nov;25(11):2239-40. doi: 10.1128/jcm.25.11.2239-2240.1987.

DOI:10.1128/jcm.25.11.2239-2240.1987
PMID:2826539
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269455/
Abstract

A high proportion of positive results was found in fecal specimens from mice and rats tested by Rotazyme II enzyme-linked immunosorbent assay, but the presence of virus could not be confirmed. Positive specimens and nonautoclaved rodent diet contained a substance that apparently attached nonspecifically to the antibody-coated beads used in the test and reacted directly with the substrate. Pretreatment of the beads with 0.1% bovine serum albumin eliminated this nonspecific activity.

摘要

通过Rotazyme II酶联免疫吸附测定法检测的小鼠和大鼠粪便样本中发现了高比例的阳性结果，但无法确认病毒的存在。阳性样本和未高压灭菌的啮齿动物饲料中含有一种物质，该物质显然非特异性地附着于测试中使用的抗体包被珠，并直接与底物发生反应。用0.1%牛血清白蛋白对珠子进行预处理可消除这种非特异性活性。