用血清白蛋白处理以消除通过Rotazyme酶联免疫吸附测定法检测的实验啮齿动物标本的假阳性结果。
Serum albumin treatment to eliminate false-positive results with laboratory rodent specimens tested by rotazyme enzyme-linked immunosorbent assay.
作者信息
Jure M N, Morse S S, Stark D M
机构信息
Laboratory Animal Research Center, Rockefeller University, New York, New York 10021-6399.
出版信息
J Clin Microbiol. 1987 Nov;25(11):2239-40. doi: 10.1128/jcm.25.11.2239-2240.1987.
Abstract
A high proportion of positive results was found in fecal specimens from mice and rats tested by Rotazyme II enzyme-linked immunosorbent assay, but the presence of virus could not be confirmed. Positive specimens and nonautoclaved rodent diet contained a substance that apparently attached nonspecifically to the antibody-coated beads used in the test and reacted directly with the substrate. Pretreatment of the beads with 0.1% bovine serum albumin eliminated this nonspecific activity.
摘要
通过Rotazyme II酶联免疫吸附测定法检测的小鼠和大鼠粪便样本中发现了高比例的阳性结果，但无法确认病毒的存在。阳性样本和未高压灭菌的啮齿动物饲料中含有一种物质，该物质显然非特异性地附着于测试中使用的抗体包被珠，并直接与底物发生反应。用0.1%牛血清白蛋白对珠子进行预处理可消除这种非特异性活性。
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