Su Jun, Zhang Erbao, Han Liang, Yin Dandan, Liu Zhili, He Xuezhi, Zhang Yuhong, Lin Feng, Lin Qingfeng, Mao Peiyao, Mao Weidong, Shen Dong
Department of Oncology, The Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, Jiangsu, China.
Department of Epidemiology and Biostatistics, Collaborative Innovation Center For Cancer Personalized Medicine, School of Public Health,Nanjing Medical University, Nanjing, China.
Cell Death Dis. 2017 Mar 9;8(3):e2665. doi: 10.1038/cddis.2017.83.
Recently, a novel class of transcripts, long noncoding RNAs (lncRNAs), is being identified at a rapid pace. These RNAs have critical roles in diverse biological processes, including tumorigenesis. One of them, BLACAT1, a cancer-associated long noncoding RNA, exerts regulatory functions in various biological processes in cancer cells, however, the role of BLACAT1 in colon cancer remains unclear. Our experiments showed that increased BLACAT1 was an independent unfavorable prognostic indicator for colorectal cancer, and revealed that BLACAT1 knockdown significantly repressed proliferation, both in vitro and in vivo. Mechanistic investigations demonstrated that BLACAT1 had a key role in G1/G0 arrest, and showed that BLACAT1 can repress p15 expression by binding to EZH2, thus contributing to the regulation of CRC cell cycle and proliferation. Our results suggest that BLACAT1, as a cell cycle regulator, may serve as a potential target for colon cancer prevention and treatment in human CRC.
最近，一类新型转录本，即长链非编码RNA（lncRNAs），正被迅速鉴定出来。这些RNA在包括肿瘤发生在内的多种生物学过程中发挥着关键作用。其中之一，BLACAT1，一种与癌症相关的长链非编码RNA，在癌细胞的各种生物学过程中发挥调节功能，然而，BLACAT1在结肠癌中的作用仍不清楚。我们的实验表明，BLACAT1表达增加是结直肠癌独立的不良预后指标，并揭示BLACAT1敲低在体外和体内均显著抑制增殖。机制研究表明，BLACAT1在G1/G0期阻滞中起关键作用，并表明BLACAT1可通过与EZH2结合抑制p15表达，从而有助于调节结直肠癌细胞周期和增殖。我们的结果表明，BLACAT1作为一种细胞周期调节因子，可能成为人类结直肠癌预防和治疗的潜在靶点。
