MOE Key Laboratory of Laser Life Science ＆ Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
MOE Key Laboratory of Laser Life Science ＆ Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Biosens Bioelectron. 2017 Aug 15;94:286-291. doi: 10.1016/j.bios.2017.02.048. Epub 2017 Mar 1.
The accurate and highly sensitive detection of prostate specific antigen (PSA) is particularly important, especially for obese men and patients. In this report, we present a novel aptamer-based surface-enhanced Raman scattering (SERS) sensor that employs magnetic nanoparticles (MNPs) core-Au nanoparticles (AuNPs) satellite assemblies to detect PSA. The high specific biorecognition between aptamer and PSA caused the dissolution of the core-satellite assemblies, thus the concentration of functionalized AuNPs (signal probes) existing in the supernatant was on the rise with the continual addition of PSA. The aptamer-modified MNPs were used as supporting materials and separation tools in the present sensor. With the assistance of magnet, the mixture was removed from the supernatant for the concentration effects. It was found that the corresponding SERS signals from the supernatant were in direct correlation to PSA concentrations over a wide range and the limit of detection (LOD) was as low as 5.0pg/mL. Excellent recovery was also obtained to assess the feasibility of this method for human serum samples detection. All of these results show a promising application of this method. And this novel sensor can be used for the accurate and highly sensitive detection of PSA in clinic samples in the future.
准确且高灵敏度地检测前列腺特异性抗原（PSA）尤为重要，尤其是对肥胖男性和患者而言。在本报告中，我们提出了一种基于适体的新型表面增强拉曼散射（SERS）传感器，该传感器采用磁性纳米颗粒（MNPs）-金纳米颗粒（AuNPs）卫星组装来检测 PSA。适体与 PSA 之间的高特异性生物识别导致了核心-卫星组装的溶解，因此随着 PSA 的不断加入，上清液中存在的功能化 AuNPs（信号探针）的浓度不断上升。在本传感器中，经适体修饰的 MNPs 被用作支撑材料和分离工具。在磁铁的帮助下，将混合物从上清液中去除以进行浓缩。结果发现，上清液中相应的 SERS 信号与 PSA 浓度在很宽的范围内呈直接相关，检测限（LOD）低至 5.0pg/mL。为了评估该方法用于人血清样品检测的可行性，还获得了良好的回收率。所有这些结果都表明该方法具有广阔的应用前景。这种新型传感器未来可用于临床样本中 PSA 的准确、高灵敏度检测。
