Jiang Xu-Ping, Tang Jing-Yuan, Xu Zhen, Han Peng, Qin Zhi-Qiang, Yang Cheng-di, Wang Shang-Qian, Tang Min, Wang Wei, Qin Chao, Xu Yang, Shen Bai-Xin, Zhou Wei-Min, Zhang Wei
Department of Urology, Affiliated Yixing Hospital of Jiangsu University, Yixing, 214200, China.
Department of Urology, State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
Environ Toxicol. 2017 Jul;32(7):1908-1917. doi: 10.1002/tox.22413. Epub 2017 Mar 15.
di-N-butylphthalate (DBP) is a ubiquitous environmental pollutant used for plastic coating and in the cosmetics industry. It has toxic effects on body health, especially the male reproductive system. Here, we investigated the effects of DBP on the male reproductive system of pubertal mice and explored the protective role of sulforaphane (SFN). The results showed that DBP significantly reduced the anogenital distance, testicular weight, sperm count and motility, and plasma and testicular testosterone levels and significantly increased the oxidative stress, sperm abnormalities, and testicular cell apoptosis. SFN supplementation ameliorated these effects. After DBP stimulation, the transcription factor nuclear factor erythroid-related factor 2 (Nrf2) was adaptively increased together with its target genes, such as HO-1 and NQO1. Upregulation of Nrf2 by SFN reduced the DBP-mediated intracellular oxidative toxicity and also increased testosterone secretion and spermatogenesis, which were decreased by DBP. These findings indicate that SFN can attenuate DBP-induced reproductive damage in pubertal mice via Nrf2-associated pathways.
邻苯二甲酸二丁酯（DBP）是一种广泛存在的环境污染物，用于塑料涂层和化妆品行业。它对人体健康有毒性作用，尤其是对男性生殖系统。在此，我们研究了DBP对青春期小鼠雄性生殖系统的影响，并探讨了萝卜硫素（SFN）的保护作用。结果表明，DBP显著缩短了肛殖距、降低了睾丸重量、精子数量和活力，以及血浆和睾丸中的睾酮水平，并显著增加了氧化应激、精子异常和睾丸细胞凋亡。补充SFN改善了这些影响。DBP刺激后，转录因子核因子红系相关因子2（Nrf2）与其靶基因如HO-1和NQO1一起适应性增加。SFN上调Nrf2可降低DBP介导的细胞内氧化毒性，还可增加睾酮分泌和精子发生，而这两者在DBP作用下均减少。这些发现表明，SFN可通过Nrf2相关途径减轻DBP诱导的青春期小鼠生殖损伤。
