Yi Ruokun, Deng Lei, Mu Jianfei, Li Chong, Tan Fang, Zhao Xin
Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, China.
Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing, China.
Front Nutr. 2021 Mar 9;8:651088. doi: 10.3389/fnut.2021.651088. eCollection 2021.
Antarctic ice microalgae ( sp.) are a polysaccharide-rich natural marine resource. In this study, we evaluated the impact of Antarctic ice microalgae polysaccharides (AIMP) on D-galactose-induced oxidation in mice. We conducted biological and biochemical tests on tissue and serum samples from mice treated with AIMP. We found that AIMP administration was associated with improved thymus, brain, heart, liver, spleen, and kidney index values. We also found that AIMP treatment inhibited the reduced aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, superoxide dismutase, glutathione peroxidase, and glutathione levels as well as the increased serum, splenic, and hepatic nitric oxide and malondialdehyde levels arising from oxidation in these animals. Pathological examination revealed that AIMP also inhibited D-galactose-induced oxidative damage to the spleen, liver, and skin of these animals. AIMP was additionally found to promote the upregulation of neuronal nitric oxide synthase, endothelial nitric oxide synthase, cuprozinc-superoxide dismutase, manganese superoxide dismutase, catalase, heme oxygenase-1, nuclear factor erythroid 2-related factor 2, γ-glutamylcysteine synthetase, and NAD(P)H dehydrogenase [quinone] 1 as well as the downregulation of inducible nitric oxide synthase in these animals. High-performance liquid chromatography analysis revealed AIMP to be composed of five monosaccharides (mannitol, ribose, anhydrous glucose, xylose, and fucose). Together, these results suggest that AIMP can effectively inhibit oxidative damage more readily than vitamin C in mice with D-galactose-induced oxidative damage, which underscores the value of developing AIMP derivatives for food purposes.
南极冰藻（属）是一种富含多糖的天然海洋资源。在本研究中，我们评估了南极冰藻多糖（AIMP）对D - 半乳糖诱导的小鼠氧化损伤的影响。我们对用AIMP处理的小鼠的组织和血清样本进行了生物学和生化测试。我们发现，给予AIMP与胸腺、脑、心脏、肝脏、脾脏和肾脏指数值的改善有关。我们还发现，AIMP处理抑制了这些动物因氧化而导致的天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶、超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽水平的降低，以及血清、脾脏和肝脏中一氧化氮和丙二醛水平的升高。病理检查显示，AIMP还抑制了D - 半乳糖诱导的这些动物脾脏、肝脏和皮肤的氧化损伤。此外，还发现AIMP促进了这些动物中神经元型一氧化氮合酶、内皮型一氧化氮合酶、铜锌超氧化物歧化酶、锰超氧化物歧化酶、过氧化氢酶、血红素加氧酶 - 1、核因子E2相关因子2、γ - 谷氨酰半胱氨酸合成酶和NAD(P)H脱氢酶[醌]1的上调，以及诱导型一氧化氮合酶 的下调。高效液相色谱分析显示AIMP由五种单糖（甘露醇、核糖、无水葡萄糖、木糖和岩藻糖）组成。总之，这些结果表明，在D - 半乳糖诱导氧化损伤的小鼠中，AIMP比维生素C能更有效地抑制氧化损伤，这凸显了开发用于食品目的的AIMP衍生物的价值。
