Orbán-Gyapai Orsolya, Liktor-Busa Erika, Kúsz Norbert, Stefkó Dóra, Urbán Edit, Hohmann Judit, Vasas Andrea
Department of Pharmacognosy, University of Szeged, 6720 Szeged, Hungary.
Institute of Clinical Microbiology, University of Szeged, 6725 Szeged, Hungary.
Fitoterapia. 2017 Apr;118:101-106. doi: 10.1016/j.fitote.2017.03.009. Epub 2017 Mar 11.
Plants belonging to the genus Rumex (family Polygonaceae) are used worldwide in traditional medicine for the treatment of various diseases caused by different microorganisms (e.g. bacteria-related dermatologic conditions, dysentery and enteritis). The present study focused on the antibacterial screening of Rumex species native to the Carpathian Basin, and isolation of compounds from one of the most efficient species, Rumex aquaticus. The antibacterial effects of n-hexane, chloroform and aqueous fractions of methanol extracts prepared from different parts of 14 Rumex species (R. acetosella, R. acetosa, R. alpinus, R. aquaticus, R. conglomeratus, R. crispus, R. hydrolapathum, R. obtusifolius subsp. obtusifolius, R. obtusifolius subsp. subalpinus, R. patientia, R. pulcher, R. scutatus, R. stenophyllus and R. thyrsiflorus) were investigated against Staphylococcus epidermidis, S. aureus, MRSA, Bacillus subtilis, Moraxella catarrhalis, Streptococcus pyogenes, S. pneumoniae, S. agalactiae, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae using the disc diffusion method. Mainly the n-hexane and chloroform extracts prepared from the roots of the plants displayed high antibacterial activity (inhibition zones>15mm) against one or more bacterial strains. The highly active extracts of the aerial part and root of R. aquaticus were subjected to a multistep separation procedure. 19 Compounds, among them naphthalenes (musizin, and its glucoside, torachrysone-glucoside, 2-methoxystypandrone), anthraquinones (emodin, chrysophanol, physcion, citreorosein, chrysophanol-8-O-glucoside), flavonoids (quercetin, quercetin-3,3'-dimethylether, isokaempferide, quercetin 3-O-arabinoside, quercetin 3-O-galactoside, catechin), stilbenes (resveratrol, piceid), and 1-stearoylglycerol were isolated from the plant. The antibacterial activities of isolated compounds were determined, and it was observed that especially naphthalenes exerted remarkable antibacterial effects against several bacterial strains.
酸模属（蓼科）植物在世界各地的传统医学中用于治疗由不同微生物引起的各种疾病（如细菌相关的皮肤病、痢疾和肠炎）。本研究聚焦于喀尔巴阡盆地本土酸模属植物的抗菌筛选，并从最有效的物种之一水生酸模中分离化合物。采用纸片扩散法，研究了从14种酸模属植物（小酸模、酸模、高山酸模、水生酸模、聚集成簇酸模、皱叶酸模、水蓼、钝叶酸模亚种钝叶酸模、钝叶酸模亚种亚高山酸模、巴天酸模、美丽酸模、盾叶酸模、狭叶酸模和圆锥酸模）不同部位制备的甲醇提取物的正己烷、氯仿和水相部分对表皮葡萄球菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、枯草芽孢杆菌、卡他莫拉菌、化脓性链球菌、肺炎链球菌、无乳链球菌、铜绿假单胞菌、大肠埃希菌和肺炎克雷伯菌的抗菌作用。主要是从植物根部制备的正己烷和氯仿提取物对一种或多种细菌菌株表现出高抗菌活性（抑菌圈>15mm）。对水生酸模地上部分和根部的高活性提取物进行了多步分离程序。从该植物中分离出19种化合物，其中包括萘类（缪斯因及其糖苷、托拉酮葡萄糖苷、2-甲氧基斯台潘酮）、蒽醌类（大黄素、 Chrysophanol、大黄酚、柠檬玫瑰素、 Chrysophanol-8-O-葡萄糖苷）、黄酮类（槲皮素、槲皮素-3,3'-二甲醚、异山奈酚、槲皮素3-O-阿拉伯糖苷、槲皮素3-O-半乳糖苷、儿茶素）、芪类（白藜芦醇、白藜芦醇苷）和1-硬脂酰甘油。测定了分离化合物的抗菌活性，观察到特别是萘类对几种细菌菌株具有显著的抗菌作用。
