Fekrazad Reza, Afzali Mehrad, Pasban-Aliabadi Hamzeh, Esmaeili-Mahani Saeed, Aminizadeh Maryam, Mostafavi Ali
Department of Periodontology, Laser Research Center in Medical Sciences (LRCMS), AJA University of Medical Sciences, Tehran, Iran.
International Network for Photo Medicine and Photo Dynamic Therapy (INPMPDT), Universal Scientific Education and Research Network (USERN), Tehran, Iran.
Braz Dent J. 2017 Jan-Feb;28(1):72-77. doi: 10.1590/0103-6440201700737.
Identifying new chemotherapeutic agents with fewer side effects is a major concern for scientists today. Thymus caramanicus Jalas (Lamiaceae family) is one of the species of Thymus that grows wild in different regions of Iran. Traditionally, leaves of this plant are used in the treatment of diabetes, arthritis and cancer. Here was investigated the cytotoxic property of Thymus caramanicus essential oil and extract in human oral epidermoid carcinoma KB cells. Cell viability was measured by MTT and neutral red assays. The cells were exposed to different concentrations of essential oil (0.05-1 µL/mL) and extract (25-150 µg/mL) for 24 h. Doxorubicin was used as anticancer control drug. The data showed that the essential oil (IC50=0.44 µL/mL) and extract (IC50=105 µg/mL) induce potent cytotoxic property. Surprisingly, cytotoxic effects of essential oil and extract of this plant on KB cancer cells were greater than those on normal gingival HGF1-PI1 cell line. In addition, Thymus caramanicus could potentiate the effect of doxorubicin in sub-effective concentrations. The results of the present study indicate that essential oils and extracts of Thymus caramanicus have potential anti-proliferative property on KB cells and can be used as pharmaceutical case study for oral cancer treatments.
寻找副作用更少的新型化疗药物是当今科学家们主要关注的问题。卡拉曼尼库斯百里香（唇形科）是在伊朗不同地区野生生长的百里香属植物之一。传统上，这种植物的叶子用于治疗糖尿病、关节炎和癌症。在此研究了卡拉曼尼库斯百里香精油和提取物对人口腔表皮样癌KB细胞的细胞毒性特性。通过MTT和中性红试验测量细胞活力。将细胞暴露于不同浓度的精油（0.05 - 1微升/毫升）和提取物（25 - 150微克/毫升）中24小时。阿霉素用作抗癌对照药物。数据表明，精油（IC50 = 0.44微升/毫升）和提取物（IC50 = 105微克/毫升）具有强大的细胞毒性特性。令人惊讶的是，这种植物的精油和提取物对KB癌细胞的细胞毒性作用大于对正常牙龈HGF1 - PI1细胞系的作用。此外，卡拉曼尼库斯百里香可以增强亚有效浓度阿霉素的作用。本研究结果表明，卡拉曼尼库斯百里香的精油和提取物对KB细胞具有潜在的抗增殖特性，可作为口腔癌治疗的药物案例研究。
