Grupo Xenomar, Departamento de Bioloxía, Facultade de Ciencias and CICA (Centro de Investigacións Científicas Avanzadas), Universidade da Coruña, Campus de A Zapateira, 15071 A Coruña, Spain.
Toxins (Basel). 2018 Oct 16;10(10):417. doi: 10.3390/toxins10100417.
The harmful effects of diarrhetic shellfish poisoning (DSP) toxins on mammalian cell lines have been widely assessed. Studies in bivalves suggest that mussels display a resistance to the cytogenotoxic effects of DSP toxins. Further, it seems that the bigger the exposure, the more resistant mussels become. To elucidate the early genetic response of mussels against these toxins, the digestive gland and the gill transcriptomes of after exposure (100,000 cells/L, 48 h) were de novo assembled based on the sequencing of 8 cDNA libraries obtained using an Illumina HiSeq 2000 platform. The assembly provided 95,702 contigs. A total of 2286 and 4523 differentially expressed transcripts were obtained in the digestive gland and the gill, respectively, indicating tissue-specific transcriptome responses. These transcripts were annotated and functionally enriched, showing 44 and 60 significant Pfam families in the digestive gland and the gill, respectively. Quantitative PCR (qPCR) was performed to validate the differential expression patterns of several genes related to lipid and carbohydrate metabolism, energy production, genome integrity and defense, suggesting their participation in the protective mechanism. This work provides knowledge of the early response against DSP toxins in the mussel s and useful information for further research on the molecular mechanisms of the bivalve resistance to these toxins.
腹泻性贝类毒素（DSP）对哺乳动物细胞系的有害影响已得到广泛评估。贝类研究表明，贻贝对 DSP 毒素的细胞遗传毒性具有抗性。此外，暴露量越大，贻贝的抗性就越强。为了阐明贻贝对这些毒素的早期遗传反应，根据 Illumina HiSeq 2000 平台获得的 8 个 cDNA 文库测序，对暴露后（100,000 个细胞/L，48 h）的贻贝消化腺和鳃转录组进行从头组装。组装提供了 95702 个连续序列。在消化腺和鳃中分别获得了 2286 和 4523 个差异表达的转录本，表明组织特异性转录组反应。这些转录本被注释和功能富集，在消化腺和鳃中分别有 44 和 60 个显著的 Pfam 家族。进行定量 PCR（qPCR）验证了与脂质和碳水化合物代谢、能量产生、基因组完整性和防御相关的几个基因的差异表达模式，表明它们参与了保护机制。这项工作提供了贻贝对 DSP 毒素早期反应的知识，并为进一步研究双壳类动物对这些毒素的抗性的分子机制提供了有用信息。
