Fang Lei, Tian Shan-Ming, Zhou Jie, Lin Yun-Liang, Wang Zhi-Wei, Wang Xiao
College of Biological Sciences and Technology, University of Jinan, Jinan 250022, PR China; Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, PR China.
Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, PR China.
Fitoterapia. 2016 Dec;115:173-176. doi: 10.1016/j.fitote.2016.10.012. Epub 2016 Oct 29.
Two new monoterpenoid indole alkaloids, melaxillines A (1) and B (2), together with four known alkaloids (3-6), were isolated from the roots of Melodinus axillaris. The structures were determined by extensive spectroscopic methods, including 1D and 2D-NMR spectroscopy, high resolution mass spectrometric data, and by comparison to literature data. New compounds 1-2 might be derived from a common precursor (3) by proposing their biogenetic route, and compound 1 represents the first Melodinus alkaloid featuring an ethyl acetate group skeleton at C-18. The anti-inflammatory effects of all the isolated compounds were evaluated by measuring the inhibitory ratios of β-glucuronidase release in rat polymorphonuclear leukocytes (PMNs) induced by platelet-activating factor. Compounds 1-4 showed significant anti-inflammatory activities with IC values ranging from 1.51 to 21.56μM, respectively.
从腋花马钱（Melodinus axillaris）的根部分离出两种新的单萜吲哚生物碱，即腋花马钱碱A（1）和B（2），以及四种已知生物碱（3 - 6）。通过广泛的光谱方法确定其结构，包括一维和二维核磁共振光谱、高分辨率质谱数据，并与文献数据进行比较。通过提出新化合物1 - 2的生源途径，推测它们可能源自共同的前体（3），化合物1是首个在C - 18位具有乙酸乙酯基团骨架的马钱属生物碱。通过测量血小板活化因子诱导的大鼠多形核白细胞（PMN）中β - 葡萄糖醛酸酶释放的抑制率，评估了所有分离化合物的抗炎作用。化合物1 - 4表现出显著的抗炎活性，IC值分别为1.51至21.56μM。
