Edwards S W, Hughes V, Barlow J, Bucknall R
Department of Biochemistry, University of Liverpool, U.K.
Biochem J. 1988 Feb 15;250(1):81-5. doi: 10.1042/bj2500081.
We have used rocket immunoelectrophoresis and immunoblotting to detect myeloperoxidase in synovial fluid from patients with rheumatoid arthritis. This protein was enzymatically inactive but its identity as myeloperoxidase was confirmed by comparing its subunit structure with that of the purified enzyme. When neutrophils were stimulated to secrete myeloperoxidase in vitro, a polypeptide with an apparent molecular mass of 62 kDa was detected extracellularly by immunoblotting. Neutrophils isolated from synovial fluid showed a reduced level of this 62 kDa polypeptide but it was detected extracellularly in synovial fluid by immunoblotting. Thus, we conclude that neutrophils in synovial fluid from patients with rheumatoid arthritis have been activated in vivo to secrete myeloperoxidase and propose that the products of this enzyme system can contribute to the tissue damage associated with this disease.
我们运用火箭免疫电泳和免疫印迹法检测类风湿性关节炎患者滑液中的髓过氧化物酶。这种蛋白质无酶活性，但通过将其亚基结构与纯化酶的亚基结构相比较，证实其为髓过氧化物酶。当体外刺激中性粒细胞分泌髓过氧化物酶时，通过免疫印迹法在细胞外检测到一种表观分子量为62 kDa的多肽。从滑液中分离出的中性粒细胞显示这种62 kDa多肽水平降低，但通过免疫印迹法在滑液的细胞外检测到了它。因此，我们得出结论，类风湿性关节炎患者滑液中的中性粒细胞在体内已被激活以分泌髓过氧化物酶，并提出该酶系统的产物可能导致与该疾病相关的组织损伤。
