Borkowski Andrzej, Cłapa Tomasz, Szala Mateusz, Gąsiński Arkadiusz, Selwet Marek
Faculty of Geology, University of Warsaw, Żwirki i Wigury 93, 02-089 Warsaw, Poland.
Department of General and Environmental Microbiology, Poznań University of Life Sciences, ul. Szydłowska 50, 60-656 Poznań, Poland.
Nanomaterials (Basel). 2016 Sep 13;6(9):171. doi: 10.3390/nano6090171.
We describe the synthesis of nanocomposites, based on nanofibers of silicon carbide, silver nanoparticles, and cellulose. Silver nanoparticle synthesis was achieved with chemical reduction using hydrazine by adding two different surfactants to obtain a nanocomposite with silver nanoparticles of different diameters. Determination of antibacterial activity was based on respiration tests. Enzymatic analysis indicates oxidative stress, and viability testing was conducted using an epifluorescence microscope. Strong bactericidal activity of nanocomposites was found against bacteria and , which were used in the study as typical Gram-negative and Gram-positive bacteria, respectively. It is assumed that reactive oxygen species generation was responsible for the observed antibacterial effect of the investigated materials. Due to the properties of silicon carbide nanofiber, the obtained nanocomposite may have potential use in technology related to water and air purification. Cellulose addition prevented silver nanoparticle release and probably enhanced bacterial adsorption onto aggregates of the nanocomposite material.
我们描述了基于碳化硅纳米纤维、银纳米颗粒和纤维素的纳米复合材料的合成。通过添加两种不同的表面活性剂，利用肼进行化学还原实现了银纳米颗粒的合成，以获得具有不同直径银纳米颗粒的纳米复合材料。抗菌活性的测定基于呼吸测试。酶分析表明存在氧化应激，并使用落射荧光显微镜进行了活力测试。发现纳米复合材料对细菌 和 具有很强的杀菌活性，在该研究中分别将它们用作典型的革兰氏阴性菌和革兰氏阳性菌。据推测，活性氧的产生是所研究材料观察到的抗菌效果的原因。由于碳化硅纳米纤维的特性，所获得的纳米复合材料可能在与水和空气净化相关的技术中具有潜在用途。纤维素的添加防止了银纳米颗粒的释放，并且可能增强了细菌在纳米复合材料聚集体上的吸附。
