Stucka R, Hauber J, Feldmann H
Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie, Universität München, Federal Republic of Germany.
Curr Genet. 1986;11(3):193-200. doi: 10.1007/BF00420606.
We have isolated and characterized a Ty element from a yeast cosmid library which exhibits several unusual features: it is flanked by non-homologous delta elements and directly associated with a singular delta element. A tRNA(Glu3) gene and tRNA(Cys) gene are found in conjunction with this element, located in opposite orientation on either end of it. The sequence information now available for several Ty elements has been used in a detailed comparative analysis to determine conserved features among the Ty elements, preferably between class I elements and a class II element. Highly conserved sequence motifs appear to be located at the borders of particular segments that correspond to the putative protein domains of the Tys. Furthermore, we include a comparison of the best-conserved amino acid homologies for these putative proteins of Ty elements, transposable elements from other organisms and several retroviral proviruses to confirm their close structural resemblance.
我们从酵母黏粒文库中分离并鉴定了一个Ty元件，它具有几个不同寻常的特征：其两侧是不同源的δ元件，并与一个单一的δ元件直接相关。在该元件的两端发现了一个tRNA(Glu3)基因和一个tRNA(Cys)基因，它们以相反的方向排列。目前可获得的几个Ty元件的序列信息已用于详细的比较分析，以确定Ty元件之间的保守特征，最好是在I类元件和II类元件之间。高度保守的序列基序似乎位于与Tys假定蛋白结构域相对应的特定片段的边界。此外，我们还比较了Ty元件、其他生物的转座元件和几种逆转录病毒前病毒的这些假定蛋白的最佳保守氨基酸同源性，以确认它们在结构上的密切相似性。
