Coyne J A, Felton A A, Lewontin R C
Proc Natl Acad Sci U S A. 1978 Oct;75(10):5090-3. doi: 10.1073/pnas.75.10.5090.
The esterase-5 locus of Drosophila pseudoobscura has been screened for variation by using a wide variety of electrophoretic conditions and by testing heat sensitivity. Among 50 isogenic lines from 17 populations, 21 genetic variants were found at the locus, 18 by electrophoresis and 3 by heat denaturation. Including alleles previously known, there are a total of 30 alleles at this locus in natural populations. This represents a doubling of the previous estimates of polymorphism at this locus. The study confirms that, in contrast to monomorphic loci, loci judged polymorphic under one detection procedure show large increases in polymorphism when examined extensively.
通过使用多种电泳条件并测试热敏感性，对拟暗果蝇的酯酶 - 5基因座进行了变异筛选。在来自17个种群的50个同基因系中，在该基因座发现了21个遗传变体，其中18个通过电泳发现，3个通过热变性发现。包括先前已知的等位基因，自然种群中该基因座共有30个等位基因。这是该基因座多态性先前估计值的两倍。该研究证实，与单态基因座不同，在一种检测程序下被判定为多态的基因座，在广泛检测时多态性会大幅增加。
