Akil H, Richardson D E, Barchas J D, Li C H
Proc Natl Acad Sci U S A. 1978 Oct;75(10):5170-2. doi: 10.1073/pnas.75.10.5170.
beta-Endorphin-like immunoreactivity in human ventricular cerebrospinal fluid was measured with a specific radioimmunoassay. The subjects were undergoing a surgical procedure for relief of chronic intractable pain. This procedure involved the focal stimulation of a medial thalamic site adjacent to the wall of the third ventricle. Samples were collected before and during the analgesic stimulation. No beta-endorphin-like immunoreactivity could be detected prior to stimulation, suggesting that baseline levels are below 25 fmol/ml of cerebrospinal fluid. Electrical stimulation led to substantial increases (13- to 20-fold) in immunoreactive material in every subject. These results suggest that beta-endorphin-like material can be released into the ventricular system and may contribute to the pain blockade that results from periventricular stimulation.
采用特异性放射免疫分析法测定了人脑室脑脊液中β-内啡肽样免疫反应性。受试者正在接受手术以缓解慢性顽固性疼痛。该手术包括对邻近第三脑室壁的丘脑内侧部位进行局部刺激。在镇痛刺激前和刺激期间采集样本。刺激前未检测到β-内啡肽样免疫反应性，这表明脑脊液的基线水平低于25 fmol/ml。电刺激导致每个受试者的免疫反应性物质大幅增加（13至20倍）。这些结果表明，β-内啡肽样物质可释放到脑室系统中，并可能有助于室周刺激所导致的疼痛阻滞。
