Turan Ibrahim, Demir Selim, Kilinc Kagan, Burnaz Nesibe Arslan, Yaman Serap Ozer, Akbulut Kubra, Mentese Ahmet, Aliyazicioglu Yuksel, Deger Orhan
Department of Genetic and Bioengineering, Faculty of Engineering and Natural Sciences, Gumushane University, 29100 Gumushane, Turkey; Medicinal Plants, Traditional Medicine Practice and Research Center, Gumushane University, 29100 Gumushane, Turkey.
Department of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical University, 61080 Trabzon, Turkey.
Saudi Pharm J. 2017 Feb;25(2):241-248. doi: 10.1016/j.jsps.2016.06.002. Epub 2016 Jun 20.
L. belongs to the family and is frequently used in traditional medicine. Numerous studies have investigated the antiproliferative effects of various extracts of different species, but studies involving the cytotoxic effect of extract are very limited. The purpose of this study was to evaluate the phenolic composition and antioxidant activity of dimethyl sulfoxide extract of (DEM) and to investigate, for the first time, the probable cytotoxic effect in human prostate adenocarcinoma (PC-3) cells together with the mechanism involved. Total polyphenolic contents (TPC), ferric reducing antioxidant power (FRAP) and phenolic compounds of DEM were evaluated using spectrophotometric procedures and HPLC. The cytotoxic effect of DEM on PC-3 cells was revealed using the MTT assay. Mechanisms involved in the cytotoxic effect of DEM on PC-3 cells were then investigated in terms of apoptosis, mitochondrial membrane potential and cell cycle using flow cytometry, while caspase activity was investigated using luminometric analysis. TPC and FRAP values were 20.7 ± 0.3 mg gallic acid equivalents and 48.8 ± 1.6 mg trolox equivalents per g sample, respectively. Ascorbic acid and chlorogenic acid were the major phenolic compounds detected at HPLC analysis. DEM arrested the cell cycle of PC-3 cells at the G phase, induced apoptosis via increased caspase activity and reduced mitochondrial membrane potential. Our results indicate that may be a novel candidate for the development of new natural product based therapeutic agents against prostate cancer.
L.属于该科，常用于传统医学。众多研究调查了不同物种各种提取物的抗增殖作用，但涉及L.提取物细胞毒性作用的研究非常有限。本研究的目的是评估L.二甲基亚砜提取物（DEM）的酚类成分和抗氧化活性，并首次研究其在人前列腺腺癌（PC-3）细胞中的可能细胞毒性作用及其相关机制。使用分光光度法和高效液相色谱法评估DEM的总多酚含量（TPC）、铁还原抗氧化能力（FRAP）和酚类化合物。使用MTT法揭示DEM对PC-3细胞的细胞毒性作用。然后使用流式细胞术从细胞凋亡、线粒体膜电位和细胞周期方面研究DEM对PC-3细胞细胞毒性作用的机制，同时使用发光分析研究半胱天冬酶活性。TPC和FRAP值分别为每克样品20.7±0.3毫克没食子酸当量和48.8±1.6毫克 Trolox当量。在高效液相色谱分析中检测到的主要酚类化合物是抗坏血酸和绿原酸。DEM使PC-3细胞的细胞周期停滞在G期，通过增加半胱天冬酶活性和降低线粒体膜电位诱导细胞凋亡。我们的结果表明，L.可能是开发新型天然产物基前列腺癌治疗药物的新候选物。
