Mendez-Perez Daniel, Alonso-Gutierrez Jorge, Hu Qijun, Molinas Margaux, Baidoo Edward E K, Wang George, Chan Leanne J G, Adams Paul D, Petzold Christopher J, Keasling Jay D, Lee Taek S
Joint BioEnergy Institute (JBEI), 5885 Hollis Street, 4th floor, Emeryville, California, 94608, USA.
Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California.
Biotechnol Bioeng. 2017 Aug;114(8):1703-1712. doi: 10.1002/bit.26296. Epub 2017 May 18.
Monoterpenes (C isoprenoids) are the main components of essential oils and are possible precursors for many commodity chemicals and high energy density fuels. Monoterpenes are synthesized from geranyl diphosphate (GPP), which is also the precursor for the biosynthesis of farnesyl diphosphate (FPP). FPP biosynthesis diverts the carbon flux from monoterpene production to C products and quinone biosynthesis. In this study, we tested a chromosomal mutation of Escherichia coli's native FPP synthase (IspA) to improve GPP availability for the production of monoterpenes using a heterologous mevalonate pathway. Monoterpene production at high levels required not only optimization of GPP production but also a basal level of FPP to maintain growth. The optimized strains produced two jet fuel precursor monoterpenoids 1,8-cineole and linalool at the titer of 653 mg/L and 505 mg/L, respectively, in batch cultures with 1% glucose. The engineered strains developed in this work provide useful resources for the production of high-value monoterpenes. Biotechnol. Bioeng. 2017;114: 1703-1712. © 2017 Wiley Periodicals, Inc.
单萜（C5 类异戊二烯）是精油的主要成分，并且可能是许多商品化学品和高能量密度燃料的前体。单萜由香叶基二磷酸（GPP）合成，而 GPP 也是法呢基二磷酸（FPP）生物合成的前体。FPP 的生物合成将碳通量从单萜生产转移至 C15 产物和醌类生物合成。在本研究中，我们测试了大肠杆菌天然 FPP 合酶（IspA）的染色体突变，以利用异源甲羟戊酸途径提高用于单萜生产的 GPP 可用性。高水平的单萜生产不仅需要优化 GPP 的生产，还需要一定水平的 FPP 以维持生长。在含 1%葡萄糖的分批培养中，优化后的菌株分别以 653 mg/L 和 505 mg/L 的滴度产生了两种喷气燃料前体单萜类化合物 1,8-桉叶素和芳樟醇。本研究构建的工程菌株为高价值单萜的生产提供了有用的资源。《生物技术与生物工程》2017 年；114：1703 - 1712。©2017 威利期刊公司
