Dong Xiaoxv, Lan Wei, Yin Xingbin, Yang Chunjing, Wang Wenping, Ni Jian
Beijing University of Chinese Medicine, Beijing 100029, China.
Beijing University of Chinese Medicine, Beijing 100029, China; Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi 830011, China.
Evid Based Complement Alternat Med. 2017;2017:8370584. doi: 10.1155/2017/8370584. Epub 2017 Mar 8.
A simple and sensitive HPLC-UV method has been developed for the simultaneous determination of quercetin, luteolin, and apigenin in rat plasma after oral administration of L. extract. The flow rate was set at 1.0 ml/min and the detection wavelength was kept at 350 nm. The calibration curves were linear in the range of 0.11-11.36 g/ml for quercetin, 0.11-11.20 g/ml for luteolin, and 0.11-10.60 g/ml for apigenin, respectively. The intraday and interday precisions (RSD) were less than 8.32 and 8.81%, respectively. The lower limits of quantification (LLOQ) of the three compounds were 0.11 g/ml. The mean recoveries for quercetin, luteolin, and apigenin were 99.11, 95.62, and 95.21%, respectively. Stability studies demonstrated that the three compounds were stable in the preparation and analytical process. The maximum plasma concentration () was 0.29 ± 0.06, 3.04 ± 0.60, and 0.42 ± 0.10 g/ml, respectively. The time to reach the maximum plasma concentration () was 0.79 ± 0.25, 0.42 ± 0.09, and 0.51 ± 0.13 h, respectively. The validated method was successfully applied to investigate the pharmacokinetics study of quercetin, luteolin, and apigenin in rat plasma after oral administration of extract.
已开发出一种简单且灵敏的高效液相色谱 - 紫外检测法，用于在大鼠口服L.提取物后同时测定其血浆中的槲皮素、木犀草素和芹菜素。流速设定为1.0毫升/分钟，检测波长保持在350纳米。槲皮素的校准曲线在0.11 - 11.36微克/毫升范围内呈线性，木犀草素在0.11 - 11.20微克/毫升范围内呈线性，芹菜素在0.11 - 10.60微克/毫升范围内呈线性。日内和日间精密度（相对标准偏差）分别小于8.32%和8.81%。这三种化合物的定量下限均为0.11微克/毫升。槲皮素、木犀草素和芹菜素的平均回收率分别为99.11%、95.62%和95.21%。稳定性研究表明，这三种化合物在制剂和分析过程中均稳定。最大血浆浓度（）分别为0.29±0.06、3.04±0.60和0.42±0.10微克/毫升。达到最大血浆浓度（）的时间分别为0.79±0.25、0.42±0.09和0.51±0.13小时。该验证方法成功应用于研究大鼠口服提取物后血浆中槲皮素、木犀草素和芹菜素的药代动力学。
