用于快速检测巨细胞病毒感染的壳瓶同步接种与感染法
Simultaneous seeding and infecting of shell vials for rapid detection of cytomegalovirus infection.
作者信息
Espy M J, Smith T F
出版信息
J Clin Microbiol. 1987 May;25(5):940-1. doi: 10.1128/jcm.25.5.940-941.1987.
Abstract
Fifty cytomegalovirus isolates were used to infect shell vials containing confluent MRC-5 cell monolayers and shell vials which were seeded with MRC-5 cells at the time of inoculation. All 50 of the isolates were detected by immunofluorescence in shell vials containing confluent monolayers, whereas 39 (78%) of the isolates were detected in shell vials that had been seeded and inoculated simultaneously (P less than 0.001). Preformed monolayers of cells in shell vials provide the most sensitive system for the detection of cytomegalovirus infection.
摘要
五十株巨细胞病毒分离株被用于感染装有汇合的MRC - 5细胞单层的空斑试验瓶以及在接种时接种了MRC - 5细胞的空斑试验瓶。在装有汇合单层的空斑试验瓶中，通过免疫荧光检测到了所有50株分离株，而在同时接种和接种了细胞的空斑试验瓶中检测到了39株（78%）分离株（P小于0.001）。空斑试验瓶中预先形成的细胞单层为检测巨细胞病毒感染提供了最敏感的系统。
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