Jones F, Grose C
Department of Microbiology, University of Iowa College of Medicine, Iowa City 52242.
J Virol. 1988 Aug;62(8):2701-11. doi: 10.1128/JVI.62.8.2701-2711.1988.
Varicella-zoster virus (VZV) encodes several glycoproteins which are present on both mature viral envelopes and the surfaces of infected cell membranes. Mechanisms of VZV glycoprotein transport and virion envelopment were investigated by both continuous radiolabeling and pulse-chase analyses with tritiated fucose in VZV-infected cells. We studied in detail the large cytoplasmic vacuoles which were present in infected cells but absent from uninfected cells. The specific activity in each subcellular compartment was defined by quantitative electron microscope autoradiography, using a cross-fire probability matrix analysis to more accurately assess the individual compartment demarcated by the silver grains. By these techniques, we documented a progression of activity originating in the Golgi apparatus and traveling through the post-Golgi region into virus-induced cytoplasmic vacuoles and finally to areas of the cellular membrane associated with the egress of viral particles. Significant amounts of radiolabel were not observed in the nucleus, and only low levels of radiolabel were associated with the cellular membrane not involved with the egress of viral particles. In addition, immunolabeling of Lowicryl-embedded VZV-infected cells demonstrated the presence of VZV glycoproteins within cytoplasmic vacuole membranes as well as on virion envelopes. These observations suggested that cytoplasmic vacuoles harbored VZV-specified glycoproteins and were also the predominant site of VZV virion envelopment within the infected cell. Neither enveloped nor unenveloped viral particles were observed within the Golgi apparatus itself.
水痘带状疱疹病毒（VZV）编码多种糖蛋白，这些糖蛋白存在于成熟病毒包膜以及受感染细胞膜表面。通过在VZV感染细胞中用氚化岩藻糖进行连续放射性标记和脉冲追踪分析，研究了VZV糖蛋白运输和病毒体包膜形成的机制。我们详细研究了感染细胞中存在但未感染细胞中不存在的大细胞质空泡。使用交叉火力概率矩阵分析更准确地评估由银颗粒划定的各个区室，通过定量电子显微镜放射自显影确定每个亚细胞区室中的比活性。通过这些技术，我们记录了一种活性进展，其起源于高尔基体，穿过高尔基体后区域进入病毒诱导的细胞质空泡，最终到达与病毒颗粒释放相关的细胞膜区域。在细胞核中未观察到大量放射性标记，并且仅低水平的放射性标记与不参与病毒颗粒释放的细胞膜相关。此外，对Lowicryl包埋的VZV感染细胞进行免疫标记显示，细胞质空泡膜内以及病毒体包膜上存在VZV糖蛋白。这些观察结果表明，细胞质空泡含有VZV特定的糖蛋白，也是感染细胞内VZV病毒体包膜形成的主要部位。在高尔基体本身内未观察到包膜或未包膜的病毒颗粒。
