Li Kai, Yu Jiangming, Xie Youtao, Huang Liping, Ye Xiaojian, Zheng Xuebin
Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, People's Republic of China.
J Mater Sci Mater Med. 2011 Dec;22(12):2781-9. doi: 10.1007/s10856-011-4454-9. Epub 2011 Oct 15.
Calcium silicate ceramic coatings have received considerable attention in recent years due to their excellent bioactivity and bonding strength. However, their high dissolution rates limit their practical applications. In this study, zinc incorporated calcium silicate based ceramic Ca(2)ZnSi(2)O(7) coating was prepared on Ti-6Al-4V substrate via plasma spraying technology aiming to achieve higher chemical stability and additional antibacterial activity. Chemical stability of the coating was assessed by monitoring mass loss and ion release of the coating after immersion in the Tris-HCl buffer solution and examining pH value variation of the solution. Results showed that the chemical stability of zinc incorporated coating was improved significantly. Antimicrobial activity of the Ca(2)ZnSi(2)O(7) coating was evaluated, and it was found that the coating exhibited 93% antibacterial ratio against Staphylococcus aureus. In addition, in vitro bioactivity and cytocompatibility were confirmed for the Ca(2)ZnSi(2)O(7) coating by simulated body fluid test, MC3T3-E1 cells adhesion investigation and cytotoxicity assay.
近年来，硅酸钙陶瓷涂层因其优异的生物活性和结合强度而受到广泛关注。然而，其高溶解率限制了它们的实际应用。在本研究中，通过等离子喷涂技术在Ti-6Al-4V基体上制备了锌掺杂的硅酸钙基陶瓷Ca(2)ZnSi(2)O(7)涂层，旨在实现更高的化学稳定性和额外的抗菌活性。通过监测涂层在Tris-HCl缓冲溶液中浸泡后的质量损失和离子释放，并检测溶液的pH值变化，评估了涂层的化学稳定性。结果表明，锌掺杂涂层的化学稳定性显著提高。对Ca(2)ZnSi(2)O(7)涂层的抗菌活性进行了评估，发现该涂层对金黄色葡萄球菌的抗菌率为93%。此外，通过模拟体液试验、MC3T3-E1细胞黏附研究和细胞毒性试验，证实了Ca(2)ZnSi(2)O(7)涂层的体外生物活性和细胞相容性。
