Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Infectious and Tropical Diseases Research Center, Health Research Institute; Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of MedicalSciences, Ahvaz, Iran.
Comp Immunol Microbiol Infect Dis. 2019 Aug;65:160-164. doi: 10.1016/j.cimid.2019.05.018. Epub 2019 May 25.
Blastocystis, a common intestinal protozoan of humans and animals, infected more than 1 billion people around the world. This enteric protozoan is frequently reported in both healthy individuals and patients with gastrointestinal (GI) symptoms.
Three hundred and forty-five fecal samples including 151 GI patients and 194 healthy individuals were examined by microscopy, culture and PCR-sequencing techniques to determine Blastocystis frequency and subtype (ST) variation.
The occurrence of Blastocystis was detected 56 (16.2%) and 85 (24.6%) by microscopy, culture and PCR methods, respectively. Out of the 85 positive patients, 60 (70.6%) were asymptomatic and 25 (29.4%) were symptomatic. The results of 41 successfully sequenced isolates identified 8 (19.5%), 8 (19.5%), and 25 (61.0%) ST1, ST2, and ST3, respectively.
This study has found that Blastocystis was more common in healthy individuals than GI patients. Another finding was that no correlation was found between clinical symptoms and Blastocystis STs.
芽囊原虫是一种常见的人类和动物肠道原生动物，感染了全球超过 10 亿人。这种肠道原生动物在健康个体和胃肠道（GI）症状患者中经常被报道。
检查了 345 份粪便样本，包括 151 名 GI 患者和 194 名健康个体，通过显微镜检查、培养和 PCR 测序技术来确定芽囊原虫的频率和亚型（ST）变化。
通过显微镜、培养和 PCR 方法分别检测到芽囊原虫的发生率为 56（16.2%）和 85（24.6%）。在 85 名阳性患者中，60 名（70.6%）无症状，25 名（29.4%）有症状。41 个成功测序的分离株的结果分别确定了 ST1、ST2 和 ST3 分别为 8（19.5%）、8（19.5%）和 25（61.0%）。
本研究发现芽囊原虫在健康个体中比 GI 患者更为常见。另一个发现是，临床症状与芽囊原虫 ST 之间没有相关性。
