School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 1370 Sankyuk-dong, Bukgu, 702-701 Daegu, Korea.
Division of Advanced Research and Development, SINOKOR, 12 Seongseogongdanbuk-ro 43-gil, Dalseo-gu, Daegu 704-920, Korea.
Sensors (Basel). 2017 Apr 13;17(4):856. doi: 10.3390/s17040856.
In this study, we developed a pore size/pore area-controlled optical biosensor-based anodic aluminum oxide (AAO) nanostructure. As the pore size of AAO increases, the unit cell of AAO increases, which also increases the non-pore area to which the antibody binds. The increase in the number of antibodies immobilized on the surface of the AAO enables effective detection of trace amounts of antigen, because increased antigen-antibody bonding results in a larger surface refractive index change. High sensitivity was thus achieved through amplification of the interference wave of two vertically-incident reflected waves through the localized surface plasmon resonance phenomenon. The sensitivity of the fabricated sensor was evaluated by measuring the change in wavelength with the change in the refractive index of the device surface, and sensitivity was increased with increasing pore-size and non-pore area. The sensitivity of the fabricated sensor was improved and up to 11.8 ag/mL serum amyloid A1 antigen was detected. In addition, the selectivity of the fabricated sensor was confirmed through a reaction with a heterogeneous substance, C-reactive protein antigen. By using hard anodization during fabrication of the AAO, the fabrication time of the device was reduced and the AAO chip was fabricated quickly and easily.
在本研究中，我们开发了一种孔径/孔面积可控的基于氧化铝阳极氧化（AAO）纳米结构的光学生物传感器。随着 AAO 孔径的增大，AAO 的单元晶格增大，与抗体结合的非孔面积也增大。固定在 AAO 表面的抗体数量的增加使痕量抗原的有效检测成为可能，因为增加的抗原-抗体结合导致更大的表面折射率变化。通过局部表面等离子体共振现象放大两个垂直入射反射波的干涉波，从而实现了高灵敏度。通过测量器件表面折射率变化引起的波长变化来评估所制备传感器的灵敏度，并且随着孔径和非孔面积的增大，灵敏度也随之增大。通过制造 AAO 时进行硬质阳极氧化，提高了传感器的灵敏度，可检测到高达 11.8 ag/mL 的血清淀粉样蛋白 A1 抗原。此外，通过与异质物质 C-反应蛋白抗原的反应证实了所制备传感器的选择性。通过在制造 AAO 时使用硬质阳极氧化，减少了器件的制造时间，并且可以快速轻松地制造 AAO 芯片。
