Lee Hyosun, Kim Dong-Uk, Park Sooyeon, Yoon Jung-Hoon, Ka Jong-Ok
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Antonie Van Leeuwenhoek. 2017 Jun;110(6):751-758. doi: 10.1007/s10482-017-0845-3. Epub 2017 Apr 22.
A Gram-stain negative, rod-shaped, aerobic bacterial strain, designated TA-C7e, was isolated from an agricultural soil obtained from Taean region, Korea. Phylogenetically, the strain was grouped with the genus Massilia and shared 95.33-97.97% 16S rRNA sequence similarity with the type strains of other species of the genus Massilia. The DNA-DNA hybridisation between TA-C7e and Massilia haematophila KACC 13771, Massilia suwonesis KACC 12635, and Massilia yuzhufengensis KACC 16569 revealed a low level of relatedness (<40%). The low levels of DNA-DNA hybridisation values identified strain TA-C7e as a novel species in the genus Massilia. The major fatty acids of the strain were C (37.3%) and summed feature 3 (C ω7c and/or C ω6c) (40.6%). Q-8 was identified as the only isoprenoid quinone. The polar lipids profile of the strain showed the presence of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminophospholipid and three unidentified lipids. The DNA G+C content of the strain was found to be 63.2 mol%. On the basis of the phenotypic, genotypic and chemotaxonomic characteristics, strain TA-C7e represents a novel species in the genus Massilia, for which the name Massilia chloroacetimidivorans sp. nov. (=KACC 18674 = NBRC 112463) is proposed.
从韩国泰安地区的农业土壤中分离出一株革兰氏染色阴性、杆状、需氧细菌菌株，命名为TA-C7e。系统发育分析表明，该菌株与马赛菌属归为一类，与马赛菌属其他物种的模式菌株16S rRNA序列相似性为95.33%-97.97%。TA-C7e与嗜血性马赛菌KACC 13771、水原马赛菌KACC 12635和玉珠峰马赛菌KACC 16569之间的DNA-DNA杂交显示出较低的相关性（<40%）。低水平的DNA-DNA杂交值表明菌株TA-C7e是马赛菌属中的一个新物种。该菌株的主要脂肪酸为C（37.3%）和总和特征3（C ω7c和/或C ω6c）（40.6%）。Q-8被鉴定为唯一的类异戊二烯醌。该菌株的极性脂质谱显示存在磷脂酰甘油、磷脂酰乙醇胺、二磷脂酰甘油、一种未鉴定的氨基磷脂和三种未鉴定的脂质。该菌株的DNA G+C含量为63.2 mol%。基于表型、基因型和化学分类学特征，菌株TA-C7e代表马赛菌属中的一个新物种，为此提出名为氯乙酰亚胺分解马赛菌（Massilia chloroacetimidivorans sp. nov.）（=KACC 18674 = NBRC 112463）。
