Sripan Panupan, Aukkanimart Ratchadawan, Boonmars Thidarut, Sriraj Pranee, Songsri Jiraporn, Boueroy Parichart, Khueangchaingkhwang Sukhonthip, Pumhirunroj Benjamabhorn, Artchayasawat Atchara
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Neglected, Zoonosis and Vector-Borne Disease Group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Asian Pac J Cancer Prev. 2017 Mar 1;18(3):779-782. doi: 10.22034/APJCP.2017.18.3.779.
Pepsin is common digestive enzyme used for fish digestion in the laboratory to collect trematode metacercariae. In a field study, to survey the infected fish is needed a huge yield of pepsin and it is very expensive. Therefore, our purpose of this study was to investigate the candidate enzyme from pineapple juice which has a digestive enzyme called bromelain, a mixture of proteolytic enzymes, to digest fish in order to harvest metacercariae. Fish were divided into 2 groups: one group in which metacercariae were harvested using acid pepsin as a control and other groups in which the fish was digested using fresh pineapple juices. The results showed that pineapple juice is able to digest fish similarly to pepsin. The Pattavia pineapple juice had the highest number of metacercariae similar to the control. For Trat Si Thong pineapple juice,we found the number of metacercariae was less than control. This result suggests that the Pattavia pineapple juice was optimal juice for fish digestion to metacercaria collection and can be used instread of pepsin acid.
胃蛋白酶是实验室中用于消化鱼类以收集吸虫囊蚴的常见消化酶。在一项实地研究中，要检测受感染的鱼类需要大量的胃蛋白酶，而且成本非常高。因此，本研究的目的是研究菠萝汁中的候选酶，菠萝汁含有一种名为菠萝蛋白酶的消化酶，它是一种蛋白水解酶混合物，用于消化鱼类以收获囊蚴。鱼被分为两组：一组使用酸性胃蛋白酶收获囊蚴作为对照，另一组使用新鲜菠萝汁消化鱼。结果表明，菠萝汁能够像胃蛋白酶一样消化鱼类。巴塔维亚菠萝汁收获的囊蚴数量与对照组最高。对于达叻府诗通菠萝汁，我们发现囊蚴数量少于对照组。这一结果表明，巴塔维亚菠萝汁是用于消化鱼类以收集囊蚴的最佳果汁，可替代酸性胃蛋白酶使用。
