Saravani Samira, Yari Davood, Saravani Ramin, Azadi Ahmadabadi Changiz
Department of Biology, Zabol University, Zabol 98615-538, Iran.
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167-43463, Iran.
Biomed Rep. 2017 Mar;6(3):329-334. doi: 10.3892/br.2017.856. Epub 2017 Feb 9.
Type 2 diabetes (T2D) is defined by high levels of glucose in the blood. The collagen IV level is associated with conditions of hyperglycemia and insulin resistance. Collagen type IV α3 chain (COL4A3) is a structural protein of the extracellular matrix (ECM). Matrix metallopeptidase 9 (MMP-9) is an enzyme that degrades the extracellular matrix and its activity is moderated by TIMP metallopeptidase inhibitor 1 (TIMP-1). The aim of the current study was to examine the association between genetic polymorphisms of (rs55703767), (rs17576) and (rs6609533) in patients with T2D. This case-control study was performed on 120 Iranian patients with T2D and 120 healthy individuals. Genotypes were analyzed using the amplification refractory mutation system-polymerase chain reaction technique. The findings demonstrated significant differences between genotypic and allelic distributions of (G/T) and (A/G) polymorphisms as follows: (G/T); TT vs. GG, odds ratio (OR)=0.235, 95% confidence interval (CI)=0.063-0.0802 (P=0.013) and T vs. G, OR=0.592, 95% CI=0.371-0.943 (P=0.026); (A/G); AG vs. GG, OR=2.429, 95% CI=1.232-4.820 (P=0.008) and A vs. G, OR=2.176, 95% CI=1.155-4.130 (P=0.013). No significant association was identified between (A/G) polymorphism and T2D in females and males. Thus, the genotypic and allelic distributions of (G/T) and (A/G) polymorphisms were associated with T2D. In addition, no significant association was identified in the genotypic distribution of the (A/G) gene in females and in males. Further studies in other ethnic groups are required to confirm these findings.
2型糖尿病（T2D）的定义是血液中葡萄糖水平升高。IV型胶原蛋白水平与高血糖和胰岛素抵抗状况相关。IV型胶原蛋白α3链（COL4A3）是细胞外基质（ECM）的一种结构蛋白。基质金属蛋白酶9（MMP - 9）是一种降解细胞外基质的酶，其活性受金属蛋白酶组织抑制剂1（TIMP - 1）调节。本研究的目的是检测T2D患者中（rs55703767）、（rs17576）和（rs6609533）基因多态性之间的关联。这项病例对照研究针对120名伊朗T2D患者和120名健康个体进行。使用扩增阻滞突变系统 - 聚合酶链反应技术分析基因型。研究结果表明，（G/T）和（A/G）多态性的基因型和等位基因分布存在显著差异，如下所示：（G/T）；TT与GG相比，比值比（OR）=0.235，95%置信区间（CI）=0.063 - 0.0802（P = 0.013），T与G相比，OR = 0.592，95% CI = 0.371 - 0.943（P = 0.026）；（A/G）；AG与GG相比，OR = 2.429，95% CI = 1.232 - 4.820（P = 0.008），A与G相比，OR = 2.176，95% CI = 1.155 - 4.130（P = 0.013）。未发现（A/G）多态性在女性和男性与T2D之间存在显著关联。因此，（G/T）和（A/G）多态性的基因型和等位基因分布与T2D相关。此外，未发现（A/G）基因在女性和男性的基因型分布中存在显著关联。需要在其他种族群体中进行进一步研究以证实这些发现。
