Morales Poole Jose Ricardo, Huang Sheng Feng, Xu Anlong, Bayet Justine, Pontarotti Pierre
Aix Marseille Université, CNRS, Centrale Marseille, I2M UMR 7373, équipe évolution biologique modélisation, 13453, Marseille, France.
State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Pharmaceutical Functional Genes, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, People's Republic of China.
Immunogenetics. 2017 Jun;69(6):391-400. doi: 10.1007/s00251-017-0979-5. Epub 2017 Apr 28.
RAG1 and RAG2 are essential subunits of the V(D)J recombinase required for the generation of the variability of antibodies and T cell receptors in jawed vertebrates. It was demonstrated that the amphioxus homologue of RAG1-RAG2 is encoded in an active transposon, belonging to the transposase DDE superfamily. The data provided support the possibility that the RAG transposon has been active through the deuterostome evolution and is still active in several lineages. The RAG transposon corresponds to several families present in deuterostomes. RAG1-RAG2 V(D)J recombinase evolved from one of them, partially due to the new ability of the transposon to interact with the cellular reparation machinery. Considering the fact that the RAG transposon survived millions of years in many different lineages, in multiple copies, and that DDE transposases evolved their association with proteins involved in repair mechanisms, we propose that the apparition of V(D)J recombination machinery could be a predictable genetic event.
RAG1和RAG2是有颌脊椎动物中抗体和T细胞受体变异性产生所需的V(D)J重组酶的必需亚基。已证明RAG1 - RAG2的文昌鱼同源物由一个活跃的转座子编码，属于转座酶DDE超家族。所提供的数据支持了RAG转座子在整个后口动物进化过程中一直活跃且仍在几个谱系中活跃的可能性。RAG转座子对应于后口动物中存在的几个家族。RAG1 - RAG2 V(D)J重组酶从其中一个家族进化而来，部分原因是转座子与细胞修复机制相互作用的新能力。考虑到RAG转座子在许多不同谱系中以多个拷贝存活了数百万年，并且DDE转座酶进化出了与参与修复机制的蛋白质的关联，我们提出V(D)J重组机制的出现可能是一个可预测的遗传事件。
