Panthavee Wanchai, Noda Masafumi, Danshiitsoodol Narandalai, Kumagai Takanori, Sugiyama Masanori
Department of Probiotic Science for Preventive Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University.
Institute of Food Research and Product Development, Kasetsart University.
Biol Pharm Bull. 2017;40(5):621-629. doi: 10.1248/bpb.b16-00856.
In the present study, we have obtained two exopolysaccharide (EPS)-producing thermophilic lactic acid bacteria (LAB) that were isolated from tropical fruits of Thailand. The two strains, designated LY45 and PY45, were identified as Pediococcus pentosaceus and Lactobacillus amylovorus, respectively. Both plant-derived LAB strains, which produce neutral EPSs together with the acidic one, can grow vigorously at 45°C and even at 50°C. Hyaluronidase (EC 3.2.1.35), which catalyzes the degradation of hyaluronic acid, activates an inflammatory reaction. Interestingly, EPSs produced by the LY45 and PY45 strains were found to inhibit hyaluronidase activity at the same order of IC values as did sodium cromoglicate and dipotassium glycyrrhizinate, which are well-known as anti-inflammatory agents. The LY45-derived neutral EPS consists of glucose and mannose as monosaccharide components, whereas the acidic one contains mainly mannose, together with glucose and galactose. On the other hand, although Lactobacillus amylovorus PY45 also produces neutral and acidic EPSs, the main monosaccharide in both EPSs is mannose, and glucose is a minor component. Furthermore, the PY45 strain may be probiotically and industrially useful because the microorganism can utilize starch and glycogen as carbon sources.
在本研究中，我们从泰国热带水果中分离出了两株产胞外多糖（EPS）的嗜热乳酸菌（LAB）。这两株菌分别命名为LY45和PY45，经鉴定分别为戊糖片球菌和解淀粉乳杆菌。这两株源自植物的LAB菌株，既能产生酸性EPS，也能产生中性EPS，在45℃甚至50℃时都能旺盛生长。透明质酸酶（EC 3.2.1.35）可催化透明质酸的降解，激活炎症反应。有趣的是，发现LY45和PY45菌株产生的EPS对透明质酸酶活性的抑制作用，其半数抑制浓度（IC）与作为抗炎剂而广为人知的色甘酸钠和甘草酸二钾处于同一水平。源自LY45的中性EPS由葡萄糖和甘露糖作为单糖成分组成，而酸性EPS主要含有甘露糖，还含有葡萄糖和半乳糖。另一方面，尽管解淀粉乳杆菌PY45也能产生中性和酸性EPS，但两种EPS中的主要单糖都是甘露糖，葡萄糖是次要成分。此外，PY45菌株在益生菌和工业应用方面可能具有价值，因为该微生物能够利用淀粉和糖原作为碳源。
