富血小板血浆：对大鼠脊髓损伤修复的有效治疗方法。
Platelet rich plasma: Effective treatment for repairing of spinal cord injury in rat.
作者信息
Salarinia Reza, Sadeghnia Hamid Reza, Alamdari Daryoush Hamidi, Hoseini Seyed Javad, Mafinezhad Asghar, Hosseini Mahmoud
机构信息
Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medical Biotechnology and Molecular Sciences, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran.
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Acta Orthop Traumatol Turc. 2017 May;51(3):254-257. doi: 10.1016/j.aott.2017.02.009. Epub 2017 Apr 25.
OBJECTIVE
The aim of the present study was to evaluate the effect of PRP on the repair of spinal cord injury in rat model.
MATERIAL AND METHODS
Rats were randomly divided into three groups with six rats in each group. Then, spinal cord injury was performed under general anesthesia using "weight dropping" method. Control group included rats receiving normal saline, group two received PRP 1 week after injury; group three received PRP 24 h after injury. The motor function was assessed weekly using the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale. Anterograde tracing was performed for evaluation of axon regeneration.
RESULT
Motor recovery was significantly better in the rats treated with PRP 24 h after injury than the control group. In the rats treated with PRP 1 week after injury and rats treated with PRP 24 h after injury, the average numbers of BDA-labeled axons were statistically different from the control group.
CONCLUSION
Our experimental study demonstrated positive effects of platelet rich plasma on nerve regeneration after spinal cord injury.
目的
本研究旨在评估富血小板血浆（PRP）对大鼠脊髓损伤修复的影响。
材料与方法
将大鼠随机分为三组，每组六只。然后，在全身麻醉下采用“重物坠落”法造成脊髓损伤。对照组大鼠接受生理盐水，第二组在损伤后1周接受PRP；第三组在损伤后24小时接受PRP。每周使用Basso、Beattie和Bresnahan（BBB）运动评分量表评估运动功能。进行顺行示踪以评估轴突再生。
结果
损伤后24小时接受PRP治疗的大鼠运动恢复明显优于对照组。损伤后1周接受PRP治疗的大鼠和损伤后24小时接受PRP治疗的大鼠，BDA标记轴突的平均数量与对照组在统计学上有差异。
结论
我们的实验研究表明富血小板血浆对脊髓损伤后的神经再生有积极作用。
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