McCullough K C, Parkinson D, Crowther J R
Department of Immunology, Animal Virus Research Institute, Woking, Surrey.
Immunology. 1988 Oct;65(2):187-91.
Using isolated peritoneal adherent cells, in which monocytes and macrophages dominate, the uptake and destruction of foot-and-mouth disease virus (FMDV) was enhanced by the opsonization with mAb of particular epitope specificity. This was seen under conditions in which virus infectivity was not neutralized, as determined by in vitro assay. Activation of macrophages in vivo further enhanced the uptake of opsonized virus, presumably by increasing the percentage of phagocytosing cells. The enhanced phagocytosis required opsonization and apparently made use of FcR+ cells, because pepsin-treated antibodies and separated F(ab')2 fragments did not enhance the capacity of the peritoneal cells to react with the virus. The reaction also relied on active phagocytosis, because inhibition of phagocytosis using silica interfered with the binding of both virus alone and virus/antibody complexes. This evidence shows that the previous in vivo observations (McCullough et al., 1986b) of enhanced protection by the mAb can be related to active phagocytosis of virus and virus/antibody complexes. The reaction is not passive adsorption to the monocyte surface, but an active phagocytosis of the virus or the complex.
使用单核细胞和巨噬细胞占主导的分离腹膜黏附细胞，特定表位特异性单克隆抗体的调理作用增强了口蹄疫病毒（FMDV）的摄取和破坏。如体外试验所确定的，在病毒感染性未被中和的条件下观察到了这种情况。体内巨噬细胞的激活进一步增强了调理素化病毒的摄取，推测是通过增加吞噬细胞的百分比实现的。增强的吞噬作用需要调理作用，并且显然利用了FcR+细胞，因为经胃蛋白酶处理的抗体和分离的F(ab')2片段并未增强腹膜细胞与病毒反应的能力。该反应还依赖于活跃的吞噬作用，因为使用二氧化硅抑制吞噬作用会干扰单独病毒以及病毒/抗体复合物的结合。这一证据表明，先前体内观察到的单克隆抗体增强保护作用（McCullough等人，1986b）可能与病毒及病毒/抗体复合物的活跃吞噬作用有关。该反应不是对单核细胞表面的被动吸附，而是对病毒或复合物的活跃吞噬作用。
