Okada Masahiro, Nakai Akira, Hara Emilio Satoshi, Taguchi Tetsushi, Nakano Takayoshi, Matsumoto Takuya
Department of Biomaterials, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
Polymeric Biomaterials Group, RCFM, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Acta Biomater. 2017 Jul 15;57:404-413. doi: 10.1016/j.actbio.2017.05.014. Epub 2017 May 5.
Over the past few years, the development of novel adhesives for biological soft tissue adhesion has gained significant interest. Such adhesives should be non-toxic and biocompatible. In this study, we synthesized a novel solid adhesive using nanostructured hydroxyapatite (HAp) and evaluated its physical adhesion properties through in vitro testing with synthetic hydrogels and mouse soft tissues. The results revealed that HAp-nanoparticle dispersions and HAp-nanoparticle-assembled nanoporous plates showed efficient adhesion to hydrogels. Interestingly, the HAp plates showed different adhesive properties depending upon the shape of their nanoparticles. The HAp plate made up of 17nm-sized nanoparticles showed an adhesive strength 2.2times higher than that of the conventional fibrin glue for mouse skin tissues.
The present study indicates a new application of inorganic biomaterials (bioceramics) as a soft tissue adhesive. Organic adhesives such as fibrin glues or cyanoacrylate derivatives have been commonly used clinically. However, their limited biocompatibility and/or low adhesion strength are some drawbacks that impair their clinical application. In this study, we synthesized a novel solid adhesive with biocompatible and biodegradable HAp nanoparticles without the aid of organic molecules, and showed a rapid and strong adhesion of mouse soft tissues compared to conventional fibrin glues. Given the importance of wet adhesion in biomedicine and biotechnology applications, our results will help not only in developing an efficient approach to close incised soft tissues, but also in finding novel ways to integrate soft tissues with synthetic hydrogels (such as drug reservoirs).
在过去几年中，用于生物软组织粘连的新型粘合剂的开发引起了极大关注。此类粘合剂应无毒且具有生物相容性。在本研究中，我们使用纳米结构的羟基磷灰石（HAp）合成了一种新型固体粘合剂，并通过与合成水凝胶和小鼠软组织的体外测试评估了其物理粘附性能。结果表明，HAp纳米颗粒分散体和HAp纳米颗粒组装的纳米多孔板对水凝胶显示出有效的粘附力。有趣的是，HAp板根据其纳米颗粒的形状表现出不同的粘附性能。由17nm大小的纳米颗粒组成的HAp板对小鼠皮肤组织的粘附强度比传统纤维蛋白胶高2.2倍。
本研究表明无机生物材料（生物陶瓷）作为软组织粘合剂的新应用。临床上通常使用有机粘合剂，如纤维蛋白胶或氰基丙烯酸酯衍生物。然而，它们有限的生物相容性和/或低粘附强度是损害其临床应用的一些缺点。在本研究中，我们在不借助有机分子的情况下合成了一种具有生物相容性和可生物降解性的HAp纳米颗粒新型固体粘合剂，并且与传统纤维蛋白胶相比，对小鼠软组织表现出快速而强烈的粘附力。鉴于湿粘附在生物医学和生物技术应用中的重要性，我们的结果不仅将有助于开发一种有效的方法来闭合切开的软组织，还将有助于找到将软组织与合成水凝胶（如药物储库）整合的新方法。
