Department of Chemistry, Abdul Wali Khan University, Mardan, Pakistan.
Department of Environmental Sciences, Abdul Wali Khan University, Mardan, Pakistan.
Biomed Res Int. 2019 Jun 17;2019:2403718. doi: 10.1155/2019/2403718. eCollection 2019.
This study investigated the phytochemical characteristics and antioxidant activity in leaves, roots, stem, flower, and seed parts of The study also assessed the heavy metal (Cr, Mn, Zn, and Cu) accumulation in each part of the plant. Among the phytochemicals, alkaloids were found only in leaves while tannins, flavonoids, and phenols were present in all parts of the plant. For antioxidant activity, free radical scavenging assay for 2,2-diphenyl-1-picrylhydrazyl (DPPH) was performed using ascorbic acid as the standard. Higher activity was shown by stem extract in methanol and leaf extract in n-hexane, ethyl acetate, and chloroform. Furthermore, all the target heavy metals were detected in all plant sections with the highest concentration of Zn in leaves and Cu in stem, root, flower, and seed. Due to stronger antioxidant potential and phytochemical composition, could prove as valuable prospect in pharmaceutical formulations by taking part in the antioxidant defense system against generation of free radicals.
本研究调查了叶片、根、茎、花和种子部分的植物化学成分特征和抗氧化活性。研究还评估了植物各部分对重金属（Cr、Mn、Zn 和 Cu）的积累。在所检测的植物化学成分中，生物碱仅存在于叶片中，而单宁、类黄酮和酚类物质存在于植物的所有部分。对于抗氧化活性，使用抗坏血酸作为标准，通过 2,2-二苯基-1-苦基肼（DPPH）自由基清除试验进行评估。在甲醇中，茎提取物的活性更高，在正己烷、乙酸乙酯和氯仿中，叶提取物的活性更高。此外，所有目标重金属都在所有植物部分中被检测到，其中锌在叶片中的浓度最高，铜在茎、根、花和种子中的浓度最高。由于具有更强的抗氧化潜力和植物化学成分，通过参与抗氧化防御系统对抗自由基的产生，可能成为有价值的药物制剂前景。
