Baylie Temesgen, Kebad Assefa, Ayelgn Tiget, Tiruneh Markeshaw, Hunie Tesfa Kibur
Department of Biomedical Science, School of Medicine, Debre Markos University, Debre Markos, Ethiopia.
Department of Pharmacology, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
J Exp Pharmacol. 2023 Oct 18;15:375-389. doi: 10.2147/JEP.S426925. eCollection 2023.
Managing diabetes mellitus with currently available drugs is costly, and the chances of side effects are high, leading to further studies for new and better medications from plant sources with the affordable and lower side effects. This study aimed to evaluate the anti-diabetic effects of Linn (Solanaceae) Leaves Extract in Streptozotocin- Induced Diabetic Mice.
Male Swiss albino mice were induced into diabetes using 150mg/kg of STZ. Mice were allocated randomly into six groups, five mice per group. Group I was a normal control, Group II was Diabetic negative control, group III was Diabetic positive control, Group IV-VI were Diabetic Mice that treated with extract (100, 200 and 400 mg/kg) for 14 days. The FBG measurements were done on 0, 7th, and 14th days of treatment. After 14th day of treatment the mice were anesthetized with diethyl ether. Then, blood was drawn by cardiac puncture to assess TC, TG, LDL-C, and HDL-C. The antioxidant activity of the extract was determined using a DPPH assay. The data were entered into Epi-Data version 4.6, exported to SPSS version 26.0, and analyzed using a one-way ANOVA followed by a Tukey post hoc test. P < 0.05 was considered statistically significant.
The extract of reduced the FBG level by 19.71%, 30.27%, 40.95%, and 45.67%, respectively, for 100, 200, 400, and GLC 5 mg/kg on the 14th day of treatment. Diabetic mice treated with for 14 days showed a significant decrease in serum TC, LDL, and serum TG and a significant increase in body weight, and HDL level as compared to diabetic negative control. Antioxidant activities of the leaves extract were comparable to ascorbic acid with an IC50 of 172.79 μg/mL.
These findings revealed that the leaves extract possesses significant Anti-diabetic activities.
使用现有药物治疗糖尿病成本高昂，且副作用发生几率高，这促使人们进一步研究从植物来源获取新型、更好的药物，这些药物价格亲民且副作用较小。本研究旨在评估酸浆（茄科）叶提取物对链脲佐菌素诱导的糖尿病小鼠的抗糖尿病作用。
使用150mg/kg的链脲佐菌素诱导雄性瑞士白化小鼠患糖尿病。将小鼠随机分为六组，每组五只。第一组为正常对照组，第二组为糖尿病阴性对照组，第三组为糖尿病阳性对照组，第四至六组为用提取物（100、200和400mg/kg）治疗14天的糖尿病小鼠组。在治疗的第0、7和14天进行空腹血糖（FBG）测量。治疗第14天后，用乙醚麻醉小鼠。然后，通过心脏穿刺采血以评估总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）。使用二苯基苦味酰基自由基（DPPH）测定法测定提取物的抗氧化活性。数据录入Epi-Data 4.6版本，导出至SPSS 26.0版本，并使用单因素方差分析，随后进行Tukey事后检验进行分析。P < 0.05被认为具有统计学意义。
在治疗第14天时，酸浆提取物分别使100、200、400mg/kg剂量组以及5mg/kg葡萄糖（GLC）组的空腹血糖水平降低了19.71%、30.27%、40.95%和45.67%。与糖尿病阴性对照组相比，用酸浆提取物治疗14天的糖尿病小鼠的血清总胆固醇、低密度脂蛋白以及血清甘油三酯显著降低，体重和高密度脂蛋白水平显著升高。叶提取物的抗氧化活性与抗坏血酸相当，半数抑制浓度（IC50）为172.79μg/mL。
这些研究结果表明酸浆叶提取物具有显著的抗糖尿病活性。
