Wang Qi, Mei Jun, Xie Jing
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai 201306, China.
Animals (Basel). 2022 Jan 29;12(3):339. doi: 10.3390/ani12030339.
This study was conducted to enhance the viability and alleviate the oxidative stress response using MO for sea bass during live transport. Six experimental groups were designed, and the effects of the physiological responses of MO were evaluated in comparison with MS-222 and eugenol. The physiological stress levels, proprotein convertase subtilisin/kexin type 9 (PCSK-9), antioxidant enzyme activities, and kidney parameters of blood serum were determined. It was found that cortisol level, glucose (Glu), lactic acid (LD), heat shock proteins (HSPs), catalase (CAT), myeloperoxidase (MPO), glutathione peroxidase (GSH-Px), uric acid (UA), and urea nitrogen (BUN) in the MO-treated samples were lower than that of the control (133.72 ng/L); however, the total antioxidant capacity (T-AOC) was higher after 72 h of the simulated live transport. The ability to resist oxidative stress increased along with the increase in the MO concentration in the water during live transport, which was similar to the results of MS-222 and eugenol treatment. In conclusion, MO, acting as a kind of novel sedative and anesthetic, can be used to improve the oxidative system and survival rate during live transport. The results of this study provide a reference for enhancing animal welfare and anti-oxidative stress ability, reducing mortality and the stress response during live fish transport.
本研究旨在通过使用薄荷醇（MO）来提高海鲈在活鱼运输过程中的活力并减轻其氧化应激反应。设计了六个实验组，并与MS-222和丁香酚进行比较，评估了MO对生理反应的影响。测定了生理应激水平、前蛋白转化酶枯草溶菌素/kexin 9型（PCSK-9）、抗氧化酶活性以及血清中的肾脏参数。结果发现，经MO处理的样本中皮质醇水平、葡萄糖（Glu）、乳酸（LD）、热休克蛋白（HSPs）、过氧化氢酶（CAT）、髓过氧化物酶（MPO）、谷胱甘肽过氧化物酶（GSH-Px）、尿酸（UA）和尿素氮（BUN）均低于对照组（133.72 ng/L）；然而，在模拟活鱼运输72小时后，总抗氧化能力（T-AOC）更高。在活鱼运输过程中，抵抗氧化应激的能力随着水中MO浓度的增加而增强，这与MS-222和丁香酚处理的结果相似。总之，MO作为一种新型镇静剂和麻醉剂，可用于改善活鱼运输过程中的氧化系统和存活率。本研究结果为提高动物福利和抗氧化应激能力、降低活鱼运输过程中的死亡率和应激反应提供了参考。
