Davis J M, La Thangue N B, Taylor D L, Latchman D S, Anderson M, Tyms A S
Department of Medical Microbiology, St Mary's Hospital Medical School, London.
Genitourin Med. 1988 Oct;64(5):321-6. doi: 10.1136/sti.64.5.321.
The synthesis of cellular macromolecules is inhibited after infection with herpes simplex viruses (HSV) although certain host proteins accumulate to high concentrations as identified by monoclonal antibody TG7A. By western blotting, a polypeptide with a relative molecular weight of 90 kilodaltons was identified in cells infected with type 2 viruses and a polypeptide of 40 kilodaltons relative molecular weight in type 1 infected cells, and virus typing was confirmed by restriction enzyme analysis of viral DNA. Thirty seven clinical isolates from the genital region were subtyped as HSV type 2 and 18 from the orofacial region as type 1 by the different intracellular location of the 90 kilodalton and 40 kilodalton proteins seen on immunofluorescent staining of cells infected with HSV. Expression of these proteins has been associated with cellular transformation due to gene products of HSV or other viruses. Overexpression of the cellular proteins identified by TG7A reactivity was shown to be a marker for cells in cervical smears from patients with CIN III that appeared to be dyskaryotic. Little or no reaction was observed in squamous epithelial cells found in normal or abnormal smears.
感染单纯疱疹病毒（HSV）后，细胞大分子的合成受到抑制，不过某些宿主蛋白会积累到高浓度，如单克隆抗体TG7A所鉴定的那样。通过蛋白质印迹法，在感染2型病毒的细胞中鉴定出一种相对分子质量为90千道尔顿的多肽，在感染1型病毒的细胞中鉴定出一种相对分子质量为40千道尔顿的多肽，并且通过对病毒DNA的限制性酶切分析证实了病毒分型。通过对感染HSV的细胞进行免疫荧光染色观察到90千道尔顿和40千道尔顿蛋白质在细胞内的不同定位，将来自生殖器区域的37株临床分离株分型为HSV 2型，将来自口面部区域的18株分型为1型。这些蛋白质的表达与HSV或其他病毒的基因产物导致的细胞转化有关。TG7A反应性所鉴定的细胞蛋白的过表达被证明是CIN III患者宫颈涂片中看似核异常的细胞的一个标志物。在正常或异常涂片中发现的鳞状上皮细胞中观察到很少或没有反应。
