Giusto Gessica, Vercelli Cristina, Comino Francesco, Caramello Vittorio, Tursi Massimiliano, Gandini Marco
Department of Veterinary Sciences, University of Torino, Largo P. Braccini 2-5, Grugliasco, (TO), Italy.
BMC Complement Altern Med. 2017 May 16;17(1):266. doi: 10.1186/s12906-017-1769-1.
Honey, alone or in combination, has been used for wound healing since ancient times and has reemerged as a topic of interest in the last decade. Pectin has recently been investigated for its use in various biomedical applications such as drug delivery, skin protection, and scaffolding for cells. The aim of the present study was to develop and evaluate a pectin-honey hydrogel (PHH) as a wound healing membrane and to compare this dressing to liquid honey.
Thirty-six adult male Sprague-Dawley rats were anesthetized and a 2 × 2 cm excisional wound was created on the dorsum. Animals were randomly assigned to four groups (PHH, LH, Pec, and C): in the PHH group, the pectin-honey hydrogel was applied under a bandage on the wound; in the LH group, liquid Manuka honey was applied; in the Pec group, pectin hydrogel was applied (Pec); and in the C group, only bandage was applied to the wound. Images of the wound were taken at defined time points, and the wound area reduction rate was calculated and compared between groups.
The wound area reduction rate was faster in the PHH, LH, and Pec groups compared to the control group and was significantly faster in the PHH group. Surprisingly, the Pec group exhibited faster wound healing than the LH group, but this effect was not statistically significant.
This is the first study using pectin in combination with honey to produce biomedical hydrogels for wound treatment. The results indicate that the use of PHH is effective for promoting and accelerating wound healing.
蜂蜜单独使用或与其他物质联合使用，自古以来就被用于伤口愈合，并且在过去十年中再次成为人们关注的话题。果胶最近因其在各种生物医学应用中的用途而受到研究，如药物递送、皮肤保护和细胞支架。本研究的目的是开发并评估一种果胶 - 蜂蜜水凝胶（PHH）作为伤口愈合膜，并将这种敷料与液态蜂蜜进行比较。
36只成年雄性Sprague - Dawley大鼠麻醉后，在背部制作一个2×2厘米的切除性伤口。动物被随机分为四组（PHH、LH、Pec和C）：在PHH组中，将果胶 - 蜂蜜水凝胶敷在伤口上并包扎；在LH组中，涂抹液态麦卢卡蜂蜜；在Pec组中，涂抹果胶水凝胶；在C组中，伤口仅进行包扎。在规定时间点拍摄伤口图像，计算伤口面积减少率并在组间进行比较。
与对照组相比，PHH组、LH组和Pec组的伤口面积减少率更快，且PHH组显著更快。令人惊讶的是，Pec组的伤口愈合比LH组更快，但这种效果无统计学意义。
这是第一项使用果胶与蜂蜜联合制备用于伤口治疗的生物医学水凝胶的研究。结果表明，使用PHH对促进和加速伤口愈合有效。
