Bruemmer Kevin J, Brewer Thomas F, Chang Christopher J
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
Curr Opin Chem Biol. 2017 Aug;39:17-23. doi: 10.1016/j.cbpa.2017.04.010. Epub 2017 May 18.
Formaldehyde (FA) is a common environmental toxin but is also endogenously produced through a diverse array of essential biological processes, including mitochondrial one-carbon metabolism, metabolite oxidation, and nuclear epigenetic modifications. Its high electrophilicity enables reactivity with a wide variety of biological nucleophiles, which can be beneficial or detrimental to cellular function depending on the context. New methods that enable detection of FA in living systems can help disentangle the signal/stress dichotomy of this simplest reactive carbonyl species (RCS), and fluorescent probes for FA with high selectivity and sensitivity have emerged as promising chemical tools in this regard.
甲醛（FA）是一种常见的环境毒素，但也通过一系列重要的生物过程内源性产生，包括线粒体一碳代谢、代谢物氧化和核表观遗传修饰。其高亲电性使其能够与多种生物亲核试剂发生反应，根据具体情况，这可能对细胞功能有益或有害。能够检测活细胞系统中FA的新方法有助于厘清这种最简单的活性羰基化合物（RCS）的信号/应激二分法，在这方面，具有高选择性和灵敏度的FA荧光探针已成为很有前景的化学工具。
