Divella Rosa, Daniele Antonella, Mazzocca Antonio, Abbate Ines, Casamassima Porzia, Caliandro Cosimo, Ruggeri Eustachio, Naglieri Emanuele, Sabbà Carlo, De Luca Raffaele
Clinical Pathology Laboratory, Department of Experimantal Oncology. Giovanni Paolo II National Cancer Institute, V.Le Orazio Flacco 65, 70124 -Bari, Italy.
Interdisciplinary Department of Medicine, University of Bari School of Medicine, Piazza G. Cesare, 11, 70124 Bari, Italy.
J Cancer. 2017 Mar 25;8(6):1000-1008. doi: 10.7150/jca.17515. eCollection 2017.
ADIPOQ gene, which encode for Adiponectin (APN), is sited on chromosome 3q27 and linked to a susceptibility locus for metabolic syndrome (MetS). The ADIPOQ rs266729 G/C gene polymorphism is significantly associated with low APN levels and linked to susceptibility to develop cancer. In addition, decreased APN serum levels are linked with tumor development and progression and inversely associated with markers of inflammation. Here, we investigate the influence of APN rs266729 G/C polymorphism on adipocytokine circulating levels and their association with MetS in colorectal cancer patients (CRC). : Blood samples from 105 CRC patients (50 women and 55 men) with and without MetS were genotyped for APN rs266729 G/C polymorphism by TETRA ARMS PCR. ELISA assay was used to measure plasma levels of APN and inflammatory TNF-α cytokine. Biochemical and anthropometric parameters of MetS were also analyzed. : We found that CRC patients (N=75) with genotype rs266729G/C or carriers of G allele were associated with a significantly increased risk of MetS development (OR =2.9) compared to those with CC genotype (N=30). Also, CG/GG genotypes were associated with significantly lower plasma APN levels and higher TNF-α levels in comparison to CC genotype (P=0.034) and APN levels were decreased in relation to BMI increases (P=0.001). : Our findings show that APN rs266729 G/C polymorphism is associated with lower APN levels in CRC patients, indicating that decreased circulating levels of APN may be a determinant risk factor for CRC in MetS patients.
脂联素（APN）基因编码脂联素，位于3号染色体q27上，与代谢综合征（MetS）的易感基因座相关。脂联素基因rs266729 G/C多态性与低APN水平显著相关，并与患癌易感性有关。此外，血清APN水平降低与肿瘤发生和进展相关，与炎症标志物呈负相关。在此，我们研究了APN rs266729 G/C多态性对结直肠癌患者（CRC）脂肪细胞因子循环水平的影响及其与MetS的关系。：对105例有或无MetS的CRC患者（50例女性和55例男性）的血样进行TETRA ARMS PCR基因分型，检测APN rs266729 G/C多态性。采用ELISA法检测血浆APN和炎性TNF-α细胞因子水平。还分析了MetS的生化和人体测量参数。：我们发现，与CC基因型（N = 30）的患者相比，基因型为rs266729G/C或携带G等位基因的CRC患者（N = 75）发生MetS的风险显著增加（OR = 2.9）。此外，与CC基因型相比，CG/GG基因型的血浆APN水平显著降低，TNF-α水平升高（P = 0.034），且APN水平随BMI增加而降低（P = 0.001）。：我们的研究结果表明，APN rs266729 G/C多态性与CRC患者较低的APN水平相关，表明APN循环水平降低可能是MetS患者患CRC的决定性危险因素。
