Boaden Elizabeth, Lyons Mary, Singhrao Sim K, Dickinson Hazel, Leathley Michael, Lightbody Catherine E, McLoughlin Alison, Khan Zarine, Crean Stjohn, Smith Craig, Higham Susan, Watkins Caroline
Faculty of Health and Wellbeing, School of Nursing, University of Central Lancashire, Preston, UK.
Dementia & Neurodegenerative Diseases Research Group, College of Clinical and Biomedical Sciences, School of Dentistry, University of Central Lancashire, Preston, UK.
Gerodontology. 2017 Sep;34(3):343-356. doi: 10.1111/ger.12271. Epub 2017 May 22.
To describe the bacterial profile of the oral flora during the first 2 weeks following a stroke, examining changes in the condition of the oral cavity and infections.
Dysphagia is common after a stroke and can lead to aspiration pneumonia. Oral flora changes associated with stroke have been implicated as a possible source of bacteria that can cause systemic infections.
Seventy-seven participants were recruited over a period of 9 months. Fifty participants had a complete set of swabs from four different oral sites and a saliva sample taken at three time points over a 14-day period. Molecular identification of bacteria was performed on the pooled DNA extracted.
A total of 103 bacterial phylotypes were identified, 29 of which were not in the Human Oral Microbiome Database (HOMD). Fourteen of the twenty most common bacterial phylotypes found in the oral cavity were Streptococcal species with Streptococcus salivarius being the most common. The condition of the oral cavity worsened during the study period. Fifteen (30%) patients had at least one infection.
There appears to be huge diversity of bacterial organisms in the oral cavity of stroke patients, and as most phylotypes identified were only found in one or two participants, no particular patterns linked to infection or the condition of the oral cavity could be discerned.
描述中风后前2周口腔菌群的细菌谱，研究口腔状况的变化及感染情况。
吞咽困难在中风后很常见，可导致吸入性肺炎。与中风相关的口腔菌群变化被认为可能是导致全身感染的细菌来源。
在9个月的时间里招募了77名参与者。50名参与者在14天内的三个时间点从四个不同口腔部位采集了全套拭子和一份唾液样本。对提取的混合DNA进行细菌的分子鉴定。
共鉴定出103种细菌系统型，其中29种不在人类口腔微生物组数据库（HOMD）中。口腔中发现的20种最常见细菌系统型中有14种是链球菌属，其中唾液链球菌最为常见。在研究期间口腔状况恶化。15名（30%）患者至少发生了一次感染。
中风患者口腔中的细菌种类似乎具有巨大的多样性，并且由于鉴定出的大多数系统型仅在一两名参与者中发现，因此无法辨别出与感染或口腔状况相关的特定模式。
