Wang Cong, Yang Yaohua, Cai Qiuyin, Gao Yutang, Cai Hui, Wu Jie, Zheng Wei, Long Jirong, Shu Xiao-Ou
Division of Epidemiology, Department of Medicine, Vanderbilt Epidemiology Center, Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN, USA.
Center for Public Health Genomics, Department of Public Health Sciences, UVA Comprehensive Cancer Center, School of Medicine, University of Virginia, Charlottesville, VA, USA.
J Oral Microbiol. 2023 Oct 8;15(1):2266655. doi: 10.1080/20002297.2023.2266655. eCollection 2023.
Stroke, a leading cause of disability worldwide, has been associated with periodontitis. However, whether stroke risk is related to oral microbiota remains unknown. This study aims to evaluate the associations between the oral microbiome and ischemic stroke risk.
In a case-control study of 134 case-control pairs nested within a prospective cohort study, we examined pre-diagnostic oral microbiome in association with stroke risk via shotgun metagenomic sequencing. The microbial sub-community and functional profiling were performed using Latent Dirichlet Allocation and HUMAnN2. Associations of microbial diversity, sub-community structure, and individual microbial features with ischemic stroke risk were evaluated via conditional logistic regression.
Alpha and beta diversities differ significantly between cases and controls. One genus- and two species-level sub-communities were significantly associated with decreased ischemic stroke risk, with odds ratios (95% confidence intervals) of 0.52 (0.31-0.90), 0.51 (0.31-0.84), and 0.60 (0.36-0.99), respectively. These associations were potentially driven by the representative taxa in these sub-communities, ., genus and , and species and ( < 0.05). Additionally, 55 taxa, 1,237 gene families, and 90 metabolic pathways were associated with ischemic stroke risk at < 0.05.
Our study highlights the role of oral microbiota in the etiology of ischemic stroke and calls for further research.
中风是全球致残的主要原因，与牙周炎有关。然而，中风风险是否与口腔微生物群相关尚不清楚。本研究旨在评估口腔微生物组与缺血性中风风险之间的关联。
在一项嵌套于前瞻性队列研究中的134对病例对照研究中，我们通过鸟枪法宏基因组测序检查了诊断前口腔微生物组与中风风险的关系。使用潜在狄利克雷分配和HUMAnN2进行微生物亚群落和功能分析。通过条件逻辑回归评估微生物多样性、亚群落结构和个体微生物特征与缺血性中风风险的关联。
病例组和对照组之间的α和β多样性存在显著差异。一个属水平和两个种水平的亚群落与缺血性中风风险降低显著相关，优势比（95%置信区间）分别为0.52（0.31 - 0.90）、0.51（0.31 - 0.84）和0.60（0.36 - 0.99）。这些关联可能由这些亚群落中的代表性分类群驱动，即属 和 ，以及种 和 （ < 0.05）。此外，55个分类群、1237个基因家族和90条代谢途径与缺血性中风风险相关， < 0.05。
我们的研究强调了口腔微生物群在缺血性中风病因中的作用，并呼吁进一步研究。
