Nicolazzo Chiara, Busetto Gian Maria, Del Giudice Francesco, Sperduti Isabella, Giannarelli Diana, Gradilone Angela, Gazzaniga Paola, de Berardinis Ettore, Raimondi Cristina
Dipartimento Medicina Molecolare, Sapienza Università di Roma, Rome, Italy.
Dipartimento Scienze Ginecologico-Ostetriche e Scienze Urologiche, Sapienza Università di Roma, Viale Regina Elena, 324, 00161, Rome, Italy.
J Cancer Res Clin Oncol. 2017 Oct;143(10):1971-1976. doi: 10.1007/s00432-017-2449-8. Epub 2017 May 29.
Long-term follow-up study to evaluate the impact on disease-free survival and cancer-specific survival of survivin expression in tissue and CTCs from T1G3 bladder cancer patients.
The study was conducted using tumor tissue and blood samples from 54 patients with a primary diagnosis of T1G3 NMIBC. Survivin was evaluated by reverse transcription-polymerase chain reaction in tumor tissues. CTCs were isolated from blood by CELLection™ Dynabeads (Invitrogen, Carlsbad, CA, USA). Cells were lysed and cDNA was synthesized and analysed for the expression of CD45, CK8 and survivin. The endpoints of this long-termanalysis were disease-free survival, DFS and cancer-specific survival, CSS.
Here, we report that, at 9 years of median follow-up, disease-free survival and cancer-specific survival are both significantly influenced by the expression of survivin in tumor tissue (p = 0.006), by the presence of CTCs (p < 0.0001) and by the expression of survivin in CTCs (p < 0.0001).
The statistically significant impact of survivin expressing CTCs on cancer-specific survival that we observed might be interpreted as the result of the persistence of a subpopulation of highlander cells in the blood of T1G3 bladder patients over time.
进行长期随访研究，以评估T1G3膀胱癌患者组织和循环肿瘤细胞（CTCs）中生存素表达对无病生存期和癌症特异性生存期的影响。
本研究使用了54例初诊为T1G3非肌层浸润性膀胱癌（NMIBC）患者的肿瘤组织和血液样本。通过逆转录-聚合酶链反应评估肿瘤组织中的生存素。采用CELLection™ Dynabeads（美国加利福尼亚州卡尔斯巴德市英杰公司）从血液中分离CTCs。裂解细胞，合成cDNA，并分析CD45、细胞角蛋白8（CK8）和生存素的表达。本次长期分析的终点为无病生存期（DFS）和癌症特异性生存期（CSS）。
在此，我们报告，在中位随访9年时，无病生存期和癌症特异性生存期均受到肿瘤组织中生存素表达（p = 0.006）、CTCs的存在（p < 0.0001）以及CTCs中生存素表达（p < 0.0001）的显著影响。
我们观察到，表达生存素的CTCs对癌症特异性生存期具有统计学显著影响，这可能被解释为T1G3膀胱癌患者血液中一小群高表达细胞长期持续存在的结果。
