Dularay B, Badesha J S, Dieppe P A, Elson C J
Department of Pathology, Bristol University.
Ann Rheum Dis. 1990 Sep;49(9):661-4. doi: 10.1136/ard.49.9.661.
Only a minority (7/35, 20%) of synovial fluids from patients with rheumatoid arthritis (RA) and none from patients with other arthritides stimulated the oxidative response of polymorphonuclear leucocytes (PMNs). Superoxide anion generation was measured by superoxide dismutase inhibitable reduction of cytochrome c. The same synovial fluids stimulated superoxide release by PMNs regardless of their source, though they elicited a greater response from RA synovial fluid PMNs than from either RA blood PMNs or blood PMNs from normal subjects. The remaining synovial fluids failed to stimulate any of the PMNs, though some (2/10) stimulated PMNs pretreated with cytochalasin B. The stimulatory activity was removed from RA synovial fluids by protein A-Sepharose and eluted with the void volume on gel chromatography. It is considered that immunoglobulin aggregates in some RA synovial fluids may stimulate the oxidative response of PMNs in the joint space but that most do not because these fluids contain either a specific inhibitor or immunoglobulin aggregates of an inappropriate type, or both.
类风湿关节炎（RA）患者的滑液中只有少数（7/35，20%）能刺激多形核白细胞（PMN）的氧化反应，而其他关节炎患者的滑液则无此作用。通过超氧化物歧化酶抑制细胞色素c的还原反应来测定超氧阴离子的生成。相同的滑液，无论来源如何，均能刺激PMN释放超氧化物，不过与来自RA血液中的PMN或正常受试者血液中的PMN相比，RA滑液中的PMN对其产生的反应更强。其余滑液未能刺激任何PMN，不过有一些（2/10）能刺激经细胞松弛素B预处理的PMN。用蛋白A - 琼脂糖从RA滑液中去除刺激活性，并在凝胶色谱上以空体积洗脱。据认为，一些RA滑液中的免疫球蛋白聚集体可能刺激关节腔内PMN的氧化反应，但大多数滑液并非如此，因为这些滑液要么含有特异性抑制剂，要么含有不适当类型的免疫球蛋白聚集体，或者两者皆有。
