Hou Wei-Chen, Miao Xiao-Hui, Ma Lian-Jun, Bai Xiao-Xue, Liu Qun, Song Lei
Department of Neurology, The First Hospital of Jilin University, Changchun 130021, China.
Clinical Laboratory, The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China.
Afr J Tradit Complement Altern Med. 2017 Jan 13;14(2):319-324. doi: 10.21010/ajtcam.v14i2.33. eCollection 2017.
The demand for the chemopreventive drug from the plant source is increasing in recent times, owing to its various biological activities without any adverse effect. The intention of this current study was to examine the anti-glioma effect of Withaferin A (WFA) on C6 glioma cell line model.
C6 glioma cells were administrated with different concentration of WFA (50, 100, 200 and 500 μg/mL) and DMSO (control) group to examine its anti-proliferative, anti-inflammatory and pro-apoptotic activities.
Treatment with WFA showed a significant decline in the glioma cell count in a dose-dependent manner and thus proving its anti-proliferative effect. Similarly, inflammatory markers were also substantially lowered upon treatment with different concentration of WFA. However, DNA fragmentation and apoptotic markers like Caspase-3 and 9 were concomitantly enhanced after co-cultured with different concentration of WFA and thus exhibiting its cytotoxicity efficacy. Furthermore, the protein expression of Bcl2 and Bax were markedly downregulated and upregulated respectively; upon treatment with WFA on C6 glioma cells.
The outcome of this study evidently demonstrates that C6 glioma cells co-cultured with increased concentration of WFA, showed an anti-proliferative, anti-inflammatory and pro-apoptotic effect in a dose-dependent fashion.
近年来，对植物源化学预防药物的需求不断增加，这是由于其具有多种生物活性且无任何不良影响。本研究的目的是检测Withaferin A（WFA）对C6胶质瘤细胞系模型的抗胶质瘤作用。
用不同浓度的WFA（50、100、200和500μg/mL）处理C6胶质瘤细胞，并设二甲基亚砜（对照）组，以检测其抗增殖、抗炎和促凋亡活性。
WFA处理后，胶质瘤细胞计数呈剂量依赖性显著下降，从而证明其抗增殖作用。同样，用不同浓度的WFA处理后，炎症标志物也显著降低。然而，与不同浓度的WFA共培养后，DNA片段化以及半胱天冬酶-3和9等凋亡标志物同时增强，从而显示出其细胞毒性作用。此外，WFA处理C6胶质瘤细胞后，Bcl2和Bax的蛋白表达分别明显下调和上调。
本研究结果清楚地表明，与浓度增加的WFA共培养的C6胶质瘤细胞呈现出剂量依赖性的抗增殖、抗炎和促凋亡作用。
