Jafarpour Dornoush, Shekarforoush Seyed Shahram, Ghaisari Hamid Reza, Nazifi Saeed, Sajedianfard Javad, Eskandari Mohammad Hadi
Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Clinical Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
BMC Complement Altern Med. 2017 Jun 5;17(1):291. doi: 10.1186/s12906-017-1803-3.
Cadmium is a heavy metal that causes oxidative stress and has toxic effects in humans. The aim of this study was to investigate the influence of two probiotics along with a prebiotic in preventing the toxic effects of cadmium in rats.
Twenty-four male Wistar rats were randomly divided into four groups namely control, cadmium only, cadmium along with Lactobacillus plantarum (1 × 109 CFU/day) and inulin (5% of feedstuff) and cadmium along with Bacillus coagulans (1 × 109 spore/day) and inulin (5% of feedstuff). Cadmium treated groups received 200 μg/rat/day CdCl2 administered by gavage. During the 42-day experimental period, they were weighed weekly. For evaluation of changes in oxidative stress, the levels of some biochemicals and enzymes of serum including SOD, GPX, MDA, AST, ALT, total bilirubin, BUN and creatinine, and also SOD level of livers were measured at day 21 and 42 of treatment. The cadmium content of kidney and liver was determined by using atomic absorption mass spectrophotometry. Data were analyzed using analysis of variance (ANOVA) followed by Duncan's post hoc test.
Treatment of cadmium induced rats with synbiotic diets significantly improved the liver enzymes and biochemical parameters that decreased AST, ALT, total bilirubin, BUN and metal accumulation in the liver and kidney and increased body weight, serum and liver SOD values in comparison with the cadmium-treated group. No significant differences were observed with MDA and GP values between all groups (p > 0.05).
This study showed that synbiotic diets containing probiotics (L. plantarum and B. coagulans) in combination with the prebiotic (inulin) can reduce the level of cadmium in the liver and kidney, preventing their damage and recover antioxidant enzymes in acute cadmium poisoning in rat.
镉是一种重金属，可引起氧化应激并对人体产生毒性作用。本研究的目的是调查两种益生菌与一种益生元一起对预防镉对大鼠的毒性作用的影响。
将24只雄性Wistar大鼠随机分为四组，即对照组、仅镉处理组、镉与植物乳杆菌（1×10⁹CFU/天）和菊粉（饲料的5%）联合处理组、镉与凝结芽孢杆菌（1×10⁹孢子/天）和菊粉（饲料的5%）联合处理组。镉处理组通过灌胃给予200μg/大鼠/天的CdCl₂。在42天的实验期内，每周对它们进行称重。为了评估氧化应激的变化，在处理的第21天和第42天测量血清中的一些生化物质和酶的水平，包括超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）、丙二醛（MDA）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、总胆红素、尿素氮（BUN）和肌酐，以及肝脏中的SOD水平。使用原子吸收质谱法测定肾脏和肝脏中的镉含量。数据采用方差分析（ANOVA），随后进行邓肯氏事后检验进行分析。
与镉处理组相比，用合生元饮食处理镉诱导的大鼠显著改善了肝脏酶和生化参数，降低了AST、ALT、总胆红素、BUN以及肝脏和肾脏中的金属积累，并增加了体重、血清和肝脏SOD值。所有组之间的MDA和GP值没有观察到显著差异（p>0.05）。
本研究表明，含有益生菌（植物乳杆菌和凝结芽孢杆菌）与益生元（菊粉）组合的合生元饮食可以降低肝脏和肾脏中的镉水平，预防其损伤，并在大鼠急性镉中毒中恢复抗氧化酶。
