通过利用ω-羟基链烷酸酯共底物来扩大甲烷营养型细菌合成的聚羟基链烷酸酯的范围。
Expanding the range of polyhydroxyalkanoates synthesized by methanotrophic bacteria through the utilization of omega-hydroxyalkanoate co-substrates.
作者信息
Myung Jaewook, Flanagan James C A, Waymouth Robert M, Criddle Craig S
机构信息
Department of Civil and Environmental Engineering, Stanford University, Stanford, CA, 94305, USA.
Department of Civil and Environmental Engineering, Pennsylvania State University, University Park, PA, 16802, USA.
出版信息
AMB Express. 2017 Dec;7(1):118. doi: 10.1186/s13568-017-0417-y. Epub 2017 Jun 5.
The first methanotrophic syntheses of polyhydroxyalkanoates (PHAs) that contain repeating units beyond 3-hydroxybutyrate and 3-hydroxyvalerate are reported. New PHAs synthesized by methanotrophs include poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB)), poly(3-hydroxybutyrate-co-5-hydroxyvalerate-co-3-hydroxyvalerate) (P(3HB-co-5HV-co-3HV)), and poly(3-hydroxybutyrate-co-6-hydroxyhexanoate-co-4-hydroxybutyrate) (P(3HB-co-6HHx-co-4HB)). This was achieved from a pure culture of Methylocystis parvus OBBP where the primary substrate is methane and the corresponding ω-hydroxyalkanoate monomers are added as a co-substrate after the cells are subjected to nitrogen-limited conditions.
首次报道了甲烷营养菌合成含有3-羟基丁酸酯和3-羟基戊酸酯以外重复单元的聚羟基脂肪酸酯（PHA）。甲烷营养菌合成的新型PHA包括聚（3-羟基丁酸酯-co-4-羟基丁酸酯）（P(3HB-co-4HB)）、聚（3-羟基丁酸酯-co-5-羟基戊酸酯-co-3-羟基戊酸酯）（P(3HB-co-5HV-co-3HV)）和聚（3-羟基丁酸酯-co-6-羟基己酸酯-co-4-羟基丁酸酯）（P(3HB-co-6HHx-co-4HB)）。这是通过小孢甲基孢囊菌OBBP的纯培养实现的，其中主要底物是甲烷，在细胞处于氮限制条件后，相应的ω-羟基链烷酸酯单体作为共底物添加。
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