Myung Jaewook, Flanagan James C A, Waymouth Robert M, Criddle Craig S
Department of Civil and Environmental Engineering, Stanford University, Stanford, CA, 94305, USA.
Department of Civil and Environmental Engineering, Pennsylvania State University, University Park, PA, 16802, USA.
AMB Express. 2017 Dec;7(1):118. doi: 10.1186/s13568-017-0417-y. Epub 2017 Jun 5.
The first methanotrophic syntheses of polyhydroxyalkanoates (PHAs) that contain repeating units beyond 3-hydroxybutyrate and 3-hydroxyvalerate are reported. New PHAs synthesized by methanotrophs include poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB)), poly(3-hydroxybutyrate-co-5-hydroxyvalerate-co-3-hydroxyvalerate) (P(3HB-co-5HV-co-3HV)), and poly(3-hydroxybutyrate-co-6-hydroxyhexanoate-co-4-hydroxybutyrate) (P(3HB-co-6HHx-co-4HB)). This was achieved from a pure culture of Methylocystis parvus OBBP where the primary substrate is methane and the corresponding ω-hydroxyalkanoate monomers are added as a co-substrate after the cells are subjected to nitrogen-limited conditions.
首次报道了甲烷营养菌合成含有3-羟基丁酸酯和3-羟基戊酸酯以外重复单元的聚羟基脂肪酸酯（PHA）。甲烷营养菌合成的新型PHA包括聚（3-羟基丁酸酯-co-4-羟基丁酸酯）（P(3HB-co-4HB)）、聚（3-羟基丁酸酯-co-5-羟基戊酸酯-co-3-羟基戊酸酯）（P(3HB-co-5HV-co-3HV)）和聚（3-羟基丁酸酯-co-6-羟基己酸酯-co-4-羟基丁酸酯）（P(3HB-co-6HHx-co-4HB)）。这是通过小孢甲基孢囊菌OBBP的纯培养实现的，其中主要底物是甲烷，在细胞处于氮限制条件后，相应的ω-羟基链烷酸酯单体作为共底物添加。
