Department of Chemistry and the Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
J Am Soc Mass Spectrom. 2017 Sep;28(9):1919-1928. doi: 10.1007/s13361-017-1704-1. Epub 2017 Jun 7.
Image-guided mass spectrometry (MS) profiling provides a facile framework for analyzing samples ranging from single cells to tissue sections. The fundamental workflow utilizes a whole-slide microscopy image to select targets of interest, determine their spatial locations, and subsequently perform MS analysis at those locations. Improving upon prior reported methodology, a software package was developed for working with microscopy images. microMS, for microscopy-guided mass spectrometry, allows the user to select and profile diverse samples using a variety of target patterns and mass analyzers. Written in Python, the program provides an intuitive graphical user interface to simplify image-guided MS for novice users. The class hierarchy of instrument interactions permits integration of new MS systems while retaining the feature-rich image analysis framework. microMS is a versatile platform for performing targeted profiling experiments using a series of mass spectrometers. The flexibility in mass analyzers greatly simplifies serial analyses of the same targets by different instruments. The current capabilities of microMS are presented, and its application for off-line analysis of single cells on three distinct instruments is demonstrated. The software has been made freely available for research purposes. Graphical Abstract ᅟ.
图像引导质谱（MS）分析为分析从单细胞到组织切片的各种样本提供了一个简单的框架。该方法的基本工作流程利用全玻片显微镜图像来选择感兴趣的目标，确定其空间位置，然后在这些位置进行 MS 分析。本研究在先前报道的方法的基础上，开发了一个用于处理显微镜图像的软件包。microMS 是用于显微镜引导 MS 的软件，它允许用户使用各种目标模式和质谱仪对多种样本进行选择和分析。该程序是用 Python 编写的，提供了一个直观的图形用户界面，使新手用户可以简化图像引导 MS。仪器交互的类层次结构允许集成新的 MS 系统，同时保留功能丰富的图像分析框架。microMS 是一个多功能平台，可用于使用一系列质谱仪进行靶向分析实验。质谱仪的灵活性极大地简化了不同仪器对同一目标的串行分析。展示了 microMS 的当前功能，并演示了其在三个不同仪器上对单细胞进行离线分析的应用。该软件已免费提供用于研究目的。
