从热带植物乌桕 Sapium ellipticum 中分离得到的内生真菌 Cylindrocarpon sp. 的代谢产物。

Metabolites from the endophytic fungus Cylindrocarpon sp. isolated from tropical plant Sapium ellipticum.

机构信息

Institute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine-University Düsseldorf, Universitätsstrasse 1, Düsseldorf 40225, Germany; Department of Organic Chemistry, Higher Teachers' Training College, University of Yaounde I, P. O. Box 47, Yaounde, Cameroon.

Department of Organic Chemistry, Higher Teachers' Training College, University of Yaounde I, P. O. Box 47, Yaounde, Cameroon.

出版信息

Fitoterapia. 2018 Jul;128:175-179. doi: 10.1016/j.fitote.2018.05.020. Epub 2018 May 17.

DOI:10.1016/j.fitote.2018.05.020

PMID:29778573

Abstract

Three new polyketides, cylindrocarpones A-C (1-3), two new pyridone alkaloids, cylindrocarpyridones A-B (5-6), a new pyrone cylindropyrone (7), together with seven know compounds were isolated from the endophytic fungus, Cylindrocarpon sp., obtained from the tropical plant Sapium ellipticum. The structures of the new compounds were elucidated by extensive analysis of their spectroscopic data (1D and 2D NMR, HRESIMS). The absolute configuration of 19-O-methyl-pyrrocidine B (13) was confirmed by X-ray analysis. All isolated compounds were screened for their cytotoxic and antibacterial activities. Pyrrocidine A (12) exhibited potent cytotoxicity against the human ovarian cancer cell line A2780 with an IC value of 1.7 μM. 19-O-Methyl-pyrrocidine B (13) showed moderate antibacterial activity against S. aureus ATCC25923 and ATCC700699 with MIC values of 50 and 25 μM, respectively.

摘要

从热带植物乌桕（ Sapium ellipticum ）中内生真菌柱孢（ Cylindrocarpon ）中分离得到三个新的聚酮化合物，柱孢酮 A-C（1-3），两个新的吡啶酮生物碱，柱孢吡咯酮 A-B（5-6），一个新的吡喃酮化合物圆柱吡喃酮（7），以及七个已知化合物。通过对其光谱数据（1D 和 2D NMR、HRESIMS）的广泛分析，阐明了新化合物的结构。通过 X 射线分析确定了 19-O-甲基-吡咯啶 B（13）的绝对构型。对所有分离得到的化合物进行了细胞毒性和抗菌活性筛选。吡咯啶 A（12）对人卵巢癌细胞 A2780 具有很强的细胞毒性，IC 值为 1.7μM。19-O-甲基-吡咯啶 B（13）对金黄色葡萄球菌 ATCC25923 和 ATCC700699 具有中度的抗菌活性，MIC 值分别为 50 和 25μM。