School of Ocean Sciences, China University of Geosciences, Beijing, 100083, People's Republic of China.
Ministry of Natural Resources, National Deep Sea Center, Qingdao, 266237, People's Republic of China.
Curr Microbiol. 2024 Aug 21;81(10):318. doi: 10.1007/s00284-024-03821-7.
Two endophytic bacterial strains, designated S1-1-2 and S1-1-8, were isolated from the leaves of a mangrove plant, Avicennia marina. The isolates were Gram-stain-negative, motile, rod-shaped bacteria with lateral flagella. Growth occurred at 4-41 °C, pH 4.0-11.0, and 0.5-25.0% NaCl. The predominant fatty acids of the novel strains were C ω6c/ω7c, C cyclo ω8c, and C. The predominant respiratory quinone was Q-9. The DNA G + C contents of strains S1-1-2 and S1-1-8 analyzed by genome sequences were 63.8%. Phylogenetic analysis based on 16S rRNA gene sequences obtained using sanger sequencing and whole-genome phylogenetic analysis revealed an affiliation between the two strains and the genus Salinicola in the class Gammaproteobacteria. Detailed genotypic, chemotaxonomic, and phenotypic data support the conclusion that these two strains should be described as a novel species in the genus Salinicola. Here, Salinicola avicenniae sp. nov. (type strain S1-1-2 = LMG 32655 = MCCC 1A19027) is proposed.
从红树林植物海桑的叶片中分离到两株内生细菌菌株，命名为 S1-1-2 和 S1-1-8。这些分离物革兰氏染色阴性，可运动，呈杆状，有侧鞭毛。它们在 4-41°C、pH4.0-11.0 和 0.5-25.0%NaCl 的条件下生长。新型菌株的主要脂肪酸为 C ω6c/ω7c、C 环 ω8c 和 C。主要呼吸醌为 Q-9。通过基因组序列分析，菌株 S1-1-2 和 S1-1-8 的 DNA G+C 含量分别为 63.8%。基于 sanger 测序获得的 16S rRNA 基因序列和全基因组系统发育分析的系统发育分析表明，这两株菌株与γ变形菌纲的 Salinicola 属有关。详细的基因型、化学生态型和表型数据支持这两个菌株应被描述为 Salinicola 属的一个新种的结论。因此，提议将该属命名为 Salinicola avicenniae sp. nov.（模式菌株 S1-1-2=LMG 32655=MCCC 1A19027）。
