Ganeshpurkar Aditya, Saluja Ajay K
Faculty of Pharmacy, Gujarat Technological University, Ahmedabad, Gujarat, India; Shri Ram Institute of Technology- Pharmacy, Jabalpur, Madhya Pradesh, India.
A.R. College of Pharmacy, Vallabh Vidyanagar, Gujarat, India.
Chem Biol Interact. 2017 Aug 1;273:154-159. doi: 10.1016/j.cbi.2017.06.006. Epub 2017 Jun 9.
Diet and dietary intake can persuade the development, safeguard and proper functioning of immune system. Ruin, an important bioflavonoid, is abundantly found in various foodstuffs. Rutin has been acknowledged for its protective and beneficial effects on various aspects of the biological system. The present study was aimed to examine the effect of rutin on the regulation of the immune response in experimental animal models. Effect of rutin of cellular immunity was determined by delayed-type hypersensitivity (DTH) response, carbon clearance assay, leucocyte mobilization test, and cyclophosphamide-induced myelosuppression, whereas humoral immunity was analyzed by the haemagglutinating antibody (HA) titre assay. Rutin (25, 50 and 100 mg/kg, p.o.) evoked a significant increase in antibody titre in the haemagglutination test, increased immunoglobulin levels, and enhanced the delayed type hypersensitivity reaction induced by sheep red blood cells. It also significantly restored the functioning of leucocytes in cyclophosphamide treated rats and augmented phagocytic index in the carbon clearance assay. The outcomes from the present study indicate that rutin possesses sufficient potential for increasing immune activity by cellular and humoral mediated mechanisms.
饮食及膳食摄入能够促进免疫系统的发育、维护及正常运作。芦丁作为一种重要的生物类黄酮，在各类食物中大量存在。芦丁因其对生物系统各方面的保护及有益作用而得到认可。本研究旨在考察芦丁对实验动物模型免疫反应调节的影响。通过迟发型超敏反应（DTH）、碳廓清试验、白细胞动员试验及环磷酰胺诱导的骨髓抑制来测定芦丁对细胞免疫的影响，而通过血凝抗体（HA）滴度测定来分析体液免疫。芦丁（25、50和100毫克/千克，口服）在血凝试验中使抗体滴度显著升高，提高免疫球蛋白水平，并增强绵羊红细胞诱导的迟发型超敏反应。它还显著恢复了环磷酰胺处理大鼠白细胞的功能，并在碳廓清试验中提高吞噬指数。本研究结果表明，芦丁具有通过细胞和体液介导机制增强免疫活性的充分潜力。
