秦艽根中具有免疫调节活性的多糖。
Polysaccharides with immunomodulating activity from roots of Gentiana crassicaulis.
机构信息
Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, 611130 Wenjiang, PR China.
Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, College of Agronomy, Sichuan Agricultural University, 611130 Wenjiang, PR China.
出版信息
Carbohydr Polym. 2017 Sep 15;172:306-314. doi: 10.1016/j.carbpol.2017.04.049. Epub 2017 Apr 23.
Two polysaccharides, GCP-I-I and GCP-II-I, were obtained from 100°C water extracts of Gentiana crassicaulis roots by DEAE anion exchange chromatography and gel filtration. The results from methanolysis, methylation, FT-IR and NMR, indicated that these two fractions are typical pectic polysaccharides, with HG and RG-I regions and AG-I/AG-II side chains, and some of the galacturonic acid units of fraction GCP-I-I were methyl esterified. Fractions GCP-I-I and GCP-II-I, both exhibited potent complement fixation, and fraction GCP-I-I was more potent than positive control BPII. The higher complement fixation activity obtained in fraction GCP-I-I may be due to the higher Mw and/or higher amount of AG-II present in fraction GCP-I-I than fraction GCP-II-I. The polysaccharides from G. crassicaulis could be used as a potential natural immunomodulator.
从秦艽 100°C 水提取物中，通过 DEAE 阴离子交换色谱和凝胶过滤得到两种多糖 GCP-I-I 和 GCP-II-I。甲醇解、甲基化、FT-IR 和 NMR 的结果表明，这两种级分是典型的果胶多糖，具有 HG 和 RG-I 区域和 AG-I/AG-II 侧链，并且级分 GCP-I-I 中的一些半乳糖醛酸单元被甲酯化。级分 GCP-I-I 和 GCP-II-I 均表现出强烈的补体固定作用，并且级分 GCP-I-I 比阳性对照 BPII 更有效。在级分 GCP-I-I 中获得的更高的补体固定活性可能是由于级分 GCP-I-I 中存在更高的 Mw 和/或更高量的 AG-II 所致。秦艽多糖可用作潜在的天然免疫调节剂。
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